J Vet Sci.  2020 Jan;21(1):e4. 10.4142/jvs.2020.21.e4.

Rapid detection of deformed wing virus in honeybee using ultra-rapid qPCR and a DNA-chip

• Kim JM
• Lim SJ
• Kim S
• Kim M
• Kim B
• Tai TA
• Kim S
• Yoon B

• KMID: 2469019
• DOI: http://doi.org/10.4142/jvs.2020.21.e4

Abstract

Fast and accurate detection of viral RNA pathogens is important in apiculture. A polymerase chain reaction (PCR)-based detection method has been developed, which is simple, specific, and sensitive. In this study, we rapidly (in 1 min) synthesized cDNA from the RNA of deformed wing virus (DWV)-infected bees (Apis mellifera), and then, within 10 min, amplified the target cDNA by ultra-rapid qPCR. The PCR products were hybridized to a DNA-chip for confirmation of target gene specificity. The results of this study suggest that our method might be a useful tool for detecting DWV, as well as for the diagnosis of RNA virus-mediated diseases on-site.

Keyword

Ultra-rapid reverse transcription-qPCR; DNA-chip; deformed wing virus; field detection

MeSH Terms

Beekeeping
Bees
Diagnosis
DNA, Complementary
Methods
Polymerase Chain Reaction
RNA
RNA, Viral
Sensitivity and Specificity
DNA, Complementary
RNA
RNA, Viral