J Korean Acad Oral Health.  2019 Dec;43(4):173-177. 10.11149/jkaoh.2019.43.4.173.

Antifungal effects of hydrogen water on the growth of Candida albicans

  • 1Department of Preventive Dentistry, School of Dentistry, Kyungpook National University, Daegu, Korea.
  • 2Department of Dental Hygine, College of Science & Technology, Kyungpook National University, Sangju, Korea. jinha01@naver.com


This study assessed the antifungal effects of neutral electrolysis hydrogen water, an agent known for its oral anti-bacterial effects on the growth of C. albicans in vitro.
Hydrogen water, denture cleansing agent, and Listerine® were compared to a tap water control. We serially cultured C. albicans KCTC 27239 in SD media to analyze the inhibitory effect of each treatment on growth. We evaluated the data using repeated-measures ANOVA test and one-way ANOVA test, and a significant difference was defined as P < 0.05.
Hydrogen water significantly inhibited the growth, but not the biofilm formation, of C. albicans compared to tap water control at 1-, 5-, and 10-minute time points (P < 0.05).
The growth of C. albicans was slower in hydrogen water than in tap water, thereby suggesting an inhibitory effect of hydrogen water. Hydrogen water may, therefore, be utilized as an antibacterial and preventive agent without any harmful effect on denture and oral tissues.


Antifungal effect; Candida albicans; Hydrogen water

MeSH Terms

Candida albicans*
In Vitro Techniques


  • Fig. 1 Intervention growth curve of C. albicans. C. albicans growth curve of exponential growth phase.

  • Fig. 2 Antifungal effect of the various rinsing water against C. albicans according to processing time. The data were analysed by one-way ANOVA test and denoted by Tukey's post-hoc analysis. *Indicates a significant difference compared with control (P<0.05).

  • Fig. 3 Antifungal effect of the various rinsing water against C. albicans biofilm according to processing time. The data were analysed by one-way ANOVA test and denoted by Tukey's post-hoc analysis.


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