Korean J Intern Med.  2019 Nov;34(6):1244-1251. 10.3904/kjim.2017.367.

Development of an acute pancreatitis porcine model based on endoscopic retrograde infusion of contrast medium or sodium taurocholate

• Park JS
• Jeong S
• Kim JM
• Lee BH
• Kim JM
• Lee DH

• KMID: 2463482
• DOI: http://doi.org/10.3904/kjim.2017.367

Abstract

BACKGROUND/AIMS
A reproducible, endoscope-based, large animal model, of acute pancreatitis was developed to meet the need for a suitable means of preclinically testing treatments. The aim of this study was to develop an endoscope-based animal model of acute pancreatitis.
METHODS
This experimental study was conducted on six mini-pigs. The pancreatitis model was induced by infusing contrast medium (CM) or sodium taurocholate (TCA) under high pressure (100 mmHg) into the main pancreatic duct by endoscopic retrograde pancreatography. Animals were randomly allocated to three groups: a CM group, a 10% TCA group, and a 20% TCA group. Pancreatic injuries were evaluated histologically, and serum amylase and lipase levels were measured.
RESULTS
Acute pancreatitis was observed in all animals during hematologic and histologic examinations. Serum amylase and lipase levels were significantly higher (> 10 times baseline), and pancreatic edema, vacuolization of acinar cells, and hemorrhagic necrosis were observed. Severity of pancreatitis tended to be greater in the TCA groups than in the CM group as assessed using histologic scores, and degrees of pancreatitis were found to be dose-dependently related to TCA concentration.
CONCLUSIONS
The two endoscopic procedures described are effective and safe for creating a swine model of acute pancreatitis. The authors hope the described endoscopic methods will assist in the development of a suitable treatment strategy.

Keyword

Pancreatitis; Animals; Endoscopes

MeSH Terms

Acinar Cells
Amylases
Animals
Edema
Endoscopes
Hope
Lipase
Models, Animal
Necrosis
Pancreatic Ducts
Pancreatitis*
Sodium*
Swine
Taurocholic Acid*
Amylases
Lipase
Sodium
Taurocholic Acid