J Korean Radiol Soc.  1982 Sep;18(3):623-628. 10.3348/jkrs.1982.18.3.623.

Effects of x-ray irradiation on mast cells and mastocalcergy in the connective tissue

• Song HY
• Rhee SJ
• Son MH
• Choi KC

• KMID: 2463290
• DOI: http://doi.org/10.3348/jkrs.1982.18.3.623

Abstract

Experiments wee performed to observe the influence of X-ray irradiation on mast cells and mastocalcergy inrats. Animals were irradiated single dose of X-ray. X-ray irradiation was applied to the whole body in doseseither 100 rads or 150 rads(Cobalt-60 Teletheraphy Unit). One day after irradiation the rats were injected leadacetate intravenously, followed by injection of compound 48/80 in the back subcutaneously. Animals were killed bydecapitation at intervals, 1 hour, 5 hours 1 day and 6 days after subcutanous injection. Specimens of theabdominal and back skin were fixed in alcoholformol solution and stained with the following methods; H-E forobservation of pathological changes of tissues, toluidine blue for demonstration of mast cells, von kossa-azure Afor demonstration of carbonate and phosphate, and chloranilic acid for demonstration of calcium. The followingconclusions were obtained. Calciphylatic wheals are large size in the control group, medium size in 100 radsirradiation group and small size in 150 rads irradiation group. In X-ray irradiation groups the number of mastcells decreases more in the 150 rads than in the 100 rads irradiation group. In the 100 rads X-ray irradiationgroup, histochemical study of the injection sites showed that calcium impregnated to mast cell granules andconnective tissue fibers in 1 days after subcutaneous injection. The morphogenesis of this calcinosis was the samein the rat of 5 hours after subcutaneous injection of the control group. Whereas, 1 day after subcutaneousinjection in 150 rads X-ray irradiation group calcium deposited more slightly than other groups.

MeSH Terms

Animals
Calcinosis
Calcium
Carbon
Chloranil
Connective Tissue*
Injections, Subcutaneous
Mast Cells*
Morphogenesis
Rats
Skin
Tolonium Chloride
Calcium
Carbon
Chloranil
Tolonium Chloride