Korean J Parasitol.  2019 Jun;57(3):309-312. 10.3347/kjp.2019.57.3.309.

Genetic Identification of Spirometra erinaceieuropaei Spargana in Liaoning and Hubei Provinces, PR China

Affiliations
  • 1Department of Microbiology and Parasitology, College of Basic Medical Science, China Medical University, Shenyang 110122, PR. China. cmucl@126.com
  • 2Department of Inspection, 4th Affiliated Hospital of China Medical University, Shenyang 110032, PR. China.
  • 3Department of Parasitology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, PR. China.

Abstract

Spargana were collected from human and frogs in Liaoning and Hubei Provinces, China. PCR amplification and direct sequencing of A cox1 fragment was PCR-amplified from genomic DNA extracted from 7 specimens (5 from humans and 2 from frogs). The cox1 fragment (390 bp) showed 97-100% similarity to the reference sequence of S. erinaceieuropaei and 88-89% to the reference sequence of S. decipiens. There were 1-12 bases different between these worms, but no obvious genetic variation (0-3.3%) to the references. There was little difference of cox1 gene between sparganum samples of humans and frogs (1-3%). This study is the first report on S. erinaceieuropaei spargana from humans in Liaoning and Hubei Provinces.

Keyword

Spirometra erinaceieuropaei; spargana; cox1; genetic analysis

MeSH Terms

China*
DNA
Genetic Variation
Humans
Polymerase Chain Reaction
Sparganum
Spirometra*
DNA
Full Text Links
  • KJP
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2024 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr