Ann Dermatol.  2019 Jun;31(3):352-355. 10.5021/ad.2019.31.3.352.

Adiponectin Promotes Caspase-14 Expression in Normal Human Epidermal Keratinocytes

• Choi SY
• Kim MJ
• Hong JY
• Park KY
• Seo SJ

• KMID: 2444875
• DOI: http://doi.org/10.5021/ad.2019.31.3.352

Abstract

No abstract available.

MeSH Terms

Adiponectin*
Caspase 14*
Humans*
Keratinocytes*
Adiponectin
Caspase 14

Figure

• 

  Fig. 1 Protein and mRNA levels of caspase-14 were measured by (A) real time reverse transcription polymerase chain reaction (RT-PCR) and (B) Western blot. β-actin expression was used as an internal control. The interleukin (IL)-4, IL-13 and calcium were treated with or without adiponectin for 72 hours. Data are represented in graphical form and show the fold change compared with sub-confluent normal control (NC) cells. (C) The phosphorylation of mitogen-activated protein kinases (MAPKs) (p38, JNK/SAPK and ERK1/2) were induced by adiponectin and (D) the caspase-14 mRNA expression after MAPKs inhibitors analyzed by real time RT-PCR. Normal human epidermal keratinocytes (NHEKs) were treated with MAPKs inhibitors; SP600125 (SP), SB203580 (SB) and PD98059 (PD) before adiponectin treatment. For the analyzed of amino acid, cells were harvested 72 hours after the start of adiponectin and interleukin treatments. One microgram of each cell lysate was loaded onto the high-performance thin-layer chromatography plate coated with cellulose and moved by mobile phase including 1-butanol, acetic acid and water (12:3:4). (E) Amino acid spots were detected by ninhydrin and compared with serine and pyrrolidone carboxylic acid (PCA) retention factor (Rf) values. The concentration of (F) PCA and (G) serine was measured using the intensity of amino acid spots and the values of measured spots calculated from 10 independent replicate experiments. Data are presented as the mean±standard deviation of at least three independent replicate experiments (n=3). DMSO: dimethyl sulfoxide, Gly: glycine. *p<0.05, **p<0.005 vs. NC, #p<0.05, ##p<0.005 vs. IL-4 and IL-13 treated group, &&p<0.005 vs. adiponectin treated group.

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