Ann Dermatol.  2019 Apr;31(2):228-231. 10.5021/ad.2019.31.2.228.

Impairment of Hair-Inducing Capacity of Three-Dimensionally Cultured Human Dermal Papilla Cells by the Ablation of STAT5

• Seo CH
• Kwack MH
• Kim MK
• Kim JC
• Sung YK

• KMID: 2439074
• DOI: http://doi.org/10.5021/ad.2019.31.2.228

Abstract

No abstract available.

MeSH Terms

Humans*

Figure

• 

  Fig. 1 Hair-inducing capacity of three-dimensionally (3D) cultured human dermal papilla cells is impaired by the knock-down of STAT5. (A) The expression of STAT5A, STAT5B, SOCS2, and SOCS3 in 3D dermal papilla (DP) spheres was quantitatively compared with that in two-dimensionally (2D) cultured DP cells by real-time polymerase chain reaction (PCR). Data are means±standard deviation (SD) of three independent experiments using DP cells of three different patients (*p<0.01). (B) Immunofluorescence staining of phospho-STAT5 (P-STAT5) (green; upper panels) and the corresponding 4′,6-diamidino-2-phenylindole (DAPI) nuclear staining (blue; bottom panels) in DP spheres are shown. Bar=0.1 mm. (C) Hair induction assay with three kinds of DP spheres (106 cells) in combination with freshly isolated mouse epidermal cells (106 cells). Bar=1 mm. (D) Phase-contrast and fluorescence imaging of DiI and DAPI in hair follicles induced from the DP spheres. Bar=0.1 mm. (E) The expression of ALP and Wnt10b in STAT5B knock-down DP spheres was quantitatively compared with that in control DP spheres using real-time PCR. Data are means±SD of three independent experiments using DP cells of three different patients (*p<0.01, **p<0.05). The sequences of primers used are as follows: STAT5A, 5′-GTAAGGCTGTGTACACTGACAC-3′ and 5′-CATAGGGTTCACAGAGAGTCTG-3′; STAT5B, 5′-GACTCAGAAATTGGCGGCATC-3′ and 5′-CGGTGTCAAGGACTGAGTCAG-3′; SOCS2, 5′-CCCGCAGGGACTCGTTTT-3′ and 5′-TTCGCGTCCTTCCTTGAAGT-3′; SOCS3, 5′-CAGCTCCAAGAGCGAGTACCA-3′ and 5′-AGAAGCCGCTCTCCTGCAG-3′; ALP, 5′-CTGGAACCGCACGGAACT-3′ and 5′-CTGCTTGGCTTTTCCTTCATG-3′; Wnt10b, 5′-CTCTGGGATGTGTAGCCTTC-3′ and 5′-GGCTCTGGAGTTGAGAAGTG-3′; and GAPDH, 5′-TGGAAATCCCATCACCATCTTC-3′ and 5′-CGCCCCACTTGATTTTGG-3′.

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