Ann Dermatol.  2019 Feb;31(1):84-87. 10.5021/ad.2019.31.1.84.

Anti-Inflammatory and Antioxidative Effects of Alpha Lipoic Acid on Cultured Human Sebocytes

• Lee WJ
• Eun DH
• Kim SM
• Kim JY
• Jang YH
• Lee SJ

• KMID: 2430808
• DOI: http://doi.org/10.5021/ad.2019.31.1.84

Abstract

No abstract available.

MeSH Terms

Humans*
Thioctic Acid*
Thioctic Acid

Figure

• 

  Fig. 1 (A) Cell Counting Kit-8 (CCK8) assay at different concentrations of alpha-lipoic acid. (B) Protein expression of inflammatory biomarkers in cultured human sebocytes after treatment with 50 µM of alpha-lipoic acid (lipoic acid) and/or 10−7 M of dihydrotestosterone (DHT) on the enzyme-linked immunosorbent assay (ELISA). (C) Protein expression of inflammatory biomarkers in cultured human sebocytes after treatment with 50 µM of alpha-lipoic acid (lipoic acid) and/or 5 µg of lipopolysaccharide (LPS) on the ELISA. IL: interleukin, TNF: tumor necrosis factor. *p<0.05.

• 

  Fig. 2 (A) Sebum production in cultured human sebocytes after treatment with 50 µM of alpha-lipoic acid (lipoic acid) and/or 10−7 M of dihydrotestosterone (DHT). (B) Lipid peroxidation in cultured human sebocytes after treatment with 50 µM of alpha-lipoic acid (lipoic acid) and/or 10−7 M of DHT. (C) Lipid peroxidation in cultured human sebocytes after treatment with 50 µM of alpha-lipoic acid (lipoic acid) and/or 5 µg of lipopolysaccharide (LPS).

Reference

1. Bowe WP, Patel N, Logan AC. Acne vulgaris: the role of oxidative stress and the potential therapeutic value of local and systemic antioxidants. J Drugs Dermatol. 2012; 11:742–746.

2. Sahib AS, Al-Anbari HH, Raghif ARA. Oxidative stress in acne vulgaris: an important therapeutic target. J Mol Pathophysiol. 2013; 2:27–31.

3. Kovalev VM. [Complex treatment of acne with lipoic acid]. Vrach Delo. 1981; (3):108–113. Russian.

4. Li X, Yang Z, Bai Y. Fluorescence spectroscopic analysis of the interaction of papain and bromelain with l-ascorbic acid, α-tocopherol, β-carotene and astaxanthin. Int J Biol Macromol. 2018; 107:144–156.

5. Choi CW, Choi JW, Park KC, Youn SW. Facial sebum affects the development of acne, especially the distribution of inflammatory acne. J Eur Acad Dermatol Venereol. 2013; 27:301–306.

6. Chang TM, Tsen JH, Yen H, Yang TY, Huang HC. Extract from periostracum cicadae inhibits oxidative stress and inflammation induced by ultraviolet B irradiation on HaCaT keratinocytes. Evid Based Complement Alternat Med. 2017; 2017:8325049.

7. Bromfield JJ, Iacovides SM. Evaluating lipopolysaccharideinduced oxidative stress in bovine granulosa cells. J Assist Reprod Genet. 2017; 34:1619–1626.

8. Ganesan K, Tiwari M, Balachandran C, Manohar BM, Puvanakrishnan R. Estrogen and testosterone attenuate extracellular matrix loss in collagen-induced arthritis in rats. Calcif Tissue Int. 2008; 83:354–364.

9. Ripple MO, Henry WF, Rago RP, Wilding G. Prooxidant-antioxidant shift induced by androgen treatment of human prostate carcinoma cells. J Natl Cancer Inst. 1997; 89:40–48.

10. Lee WJ, Kim SL, Choe YS, Jang YH, Lee SJ, Kim DW. Magnesium ascorbyl phosphate regulates the expression of inflammatory biomarkers in cultured sebocytes. Ann Dermatol. 2015; 27:376–382.