J Korean Acad Pediatr Dent.  2018 May;45(2):144-153. 10.5933/JKAPD.2018.45.2.144.

Roles of Sonic Hedgehog Signaling During Tooth Root and Periodontium Formation

• Hwang J
• Cho ES
• Yang Y

• KMID: 2430472
• DOI: http://doi.org/10.5933/JKAPD.2018.45.2.144

Abstract

The aim of this study was to understand the roles of Sonic Hedgehog (SHH) signaling during tooth root and periodontium formation. In this study, we generated the dental mesenchyme-specific Smoothened (Smo) activated/inactivated mice with the activity of Cre recombinase under the control of osteocalcin promoter. In the Smo activated mutant molar sections at the postnatal 28 days, we found extremely thin root dentin and widened pulp chamber. Picrosirius red staining showed loosely arranged fibers in the periodontal space and decreased cellular cementum with some root resorption. Immunohistochemical staining showed less localization of matrix proteins such as Bsp, Dmp1, Pstn, and Ank in the cementum, periodontal ligament, and/or cementoblast. In the Smo inactivated mutant mouse, there was not any remarkable differences in the localization of these matrix proteins compared with the wild type. These findings suggest that adequate suppressing regulation of SHH signaling is required in the development of tooth root and periodontium.

Keyword

Sonic Hedgehog signaling; Smoothened; Periodontium

MeSH Terms

Animals
Dental Cementum
Dental Pulp Cavity
Dentin
Hedgehogs*
Mice
Molar
Osteocalcin
Periodontal Ligament
Periodontium*
Recombinases
Root Resorption
Tooth Root*
Tooth*
Osteocalcin
Recombinases