Clin Exp Reprod Med.  2018 Dec;45(4):170-176. 10.5653/cerm.2018.45.4.170.

Novel method of histopathological analysis after testicular sperm extraction in patients with nonobstructive and obstructive azoospermia

• Cito G
• Coccia ME
• Picone R
• Nesi G
• Cocci A
• Dabizzi S
• Garaffa G
• Fucci R
• Falcone P
• Bertocci F
• Santi R
• Criscuoli
• Serni S
• Carini M
• Natali A

• KMID: 2427412
• DOI: http://doi.org/10.5653/cerm.2018.45.4.170

Abstract

OBJECTIVE
To assess whether the "testicular pool" could be used for histological analysis and whether it gave more accurate information than the standard testicular biopsy.
METHODS
Between January 2017 and March 2018, this single-center prospective study included 60 azoospermic men undergoing conventional bilateral testicular sperm extraction. Six samples were excised from each testicle and transferred to an embryologist. One additional biopsy was randomly taken from each testis for a histological analysis. After processing, the testicular pool was also sent for a histological analysis, which showed normal spermatogenesis (NS), hypospermatogenesis (HYPO), maturation arrest (MA), Sertoli cell-only syndrome (SCOS), and tubular atrophy (TA).
RESULTS
Twenty of the 60 patients (33.3%) had obstructive azoospermia (OA), while the remaining 40 (66.6%) had nonobstructive azoospermia. Their mean age was 40.5 years. All patients with OA had previously undergone unsuccessful testicular fine-needle aspiration. Successful sperm retrieval (SSR) occurred in 93.3% of patients. Histological analysis of the testicular biopsy revealed NS in 12 patients (20%), HYPO and TA in 28 patients (46.6%), MA in eight patients (13.3%), and SCOS in 12 patients (20%). The testicular pool analysis showed NS in 12 patients (20%), HYPO and TA in 44 patients (73.3%), MA in four patients (6.6%), and SCOS in no patients. In four patients with MA (6.6% of the total sample) and 12 patients with SCOS (20% of the total sample) according to the standard testicular biopsy, the embryologist found SSR with cryopreservation. Overall, in 44 patients (73.3%), the testicular pool analysis confirmed the histological findings of the standard testicular biopsy. In the 16 cases (26.6%) with a discrepancy between the single-biopsy histological findings and SSR, the testicular pool analysis confirmed the embryological data on SSR.
CONCLUSION
The testicular pool proved to be easily analyzable, practical, manageable, and more accurate for predicting sperm retrieval than standard testicular biopsy.

Keyword

Azoospermia; Histology; Male infertility; Sperm retrieval; Testis

MeSH Terms

Atrophy
Azoospermia*
Biopsy
Biopsy, Fine-Needle
Cryopreservation
Humans
Infertility, Male
Male
Methods*
Oligospermia
Prospective Studies
Sertoli Cell-Only Syndrome
Sperm Retrieval
Spermatogenesis
Spermatozoa*
Testis