Nat Prod Sci.  2018 Sep;24(3):159-163. 10.20307/nps.2018.24.3.159.

Chinoketides A and B, Two New Antimicrobial Polyketides from the Endophytes of Distylium chinense with the “Black-Box” Co-culture Method

• Lv MM
• Tan MH
• Lu LW
• Zhang RH
• Guo ZY
• Liu CX
• Yang J
• Zou K
• Proksch P

• KMID: 2422007
• DOI: http://doi.org/10.20307/nps.2018.24.3.159

Abstract

Two new polyketides, chinoketides A and B (1 - 2) with a known compound xylarphthalide A (3), were isolated from the solid medium of the endophytes from the leaves of the relic plant Distylium chinense with the "black-box" co-culture method, and the structures of two new compounds were elucidated by NMR, MS and CD spectra. And the absolute configurations of chinoketides A (1) and B (2) were determined as 2R,3R,8S and 5R,6S by calculating their ECD spectra to compare with the experimental CD spectra. Finally, the antimicrobial activities were evaluated to Erwinia carotovora sub sp. Carotovora (Jones) Bersey et al, and the results showed that compounds 1 - 3 displayed the antimicrobial activities with MIC value at 20.5, 30.4 and 10.2 µg/mL.

Keyword

Polyketides; “black-box” Co-culture; Distylium chinense; Antimicrobial activity; Structural elucidation

MeSH Terms

Coculture Techniques*
Endophytes*
Methods*
Pectobacterium carotovorum
Plants
Polyketides*
Polyketides

Figure

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  Fig. 1 The structures of compounds 1 – 3.

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  Fig. 2 The key HMBC, NOESY and COSY correlations of compounds 1 – 2.

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  Fig. 3 The calculated ECD and experimental CD spectra of compound 1.

• 

  Fig. 4 The calculated ECD and experimental CD spectra of compound 2.

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