Nutr Res Pract.  2010 Feb;4(1):30-35.

The antioxidant and chemopreventive potentialities of Mosidae (Adenophora remotiflora) leaves

• Kim AJ
• Han MR
• Kim MH
• Lee M
• Yoon TJ
• Ha SD

Abstract

Our study focused on the antioxidant activities of Mosidae leaf ethanol extract (MLE) and included measurements of reducing power, total phenolic compounds, DPPH radical scavenging activity, and hydroxyl radical scavenging activity. In order to determine whether or not MLE evidences any chemopreventive activities, experimental lung metastasis was induced via the i.v. inoculation of colon26-M3.1 carcinoma cells into BALB/c mice. Additionally, we attempted to characterize any possible cytotoxic effects in murine normal splenocytes and tumor cells (B16-BL6 and colon26-M3.1). The total phenolic content and reducing capacity were measured at 39 mg/100 mL and 1.24, respectively, whereas the DPPH and hydroxyl radical scavenging activities of MLE were measured to be 88.89% and 22.10%, respectively. Prophylactic i.v. treatment with MLE resulted in a dose-dependent and significant inhibition of lung metastasis. Specifically, a MLE dose of 200 ug per mouse resulted in an 88.90% inhibition of lung metastasis. For the cytotoxicity assay, MLE doses up to 100 ug/mL were not shown to affect the growth of normal murine splenocytes. Additionally, the survival of normal cells was not affected at MLE doses below 500 ug/mL. However, MLE doses up to 500 ug/mL reduced the percentage of tumor cell growth for B16BL6 (67% alive) and colon26-M3.1 (62% alive) cells.

Keyword

Mosidae (Adenophora remotiflora) leaves; antioxidant activity; cytotoxicity assay; tumor metastasis

MeSH Terms

Animals
Ethanol
Hydroxyl Radical
Lung
Mice
Neoplasm Metastasis
Phenol
Ethanol
Hydroxyl Radical
Phenol

Figure

• 

  Fig. 1 The Mosidae (Adenophora remotiflora) leaves. Two-month-old fresh (a) and vacuum freeze dried (b) of Mosidae leaves were donated by Eco-Sprout Co, Ltd. (Gyeonggido, Korea) and identified by Dr Kang, Department of Oriental medicine Resources, Joongbu University (Keumsan, Korea).

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  Fig. 2 Cytotoxicity assays of Mosidae leaves ethanol extract (MLE) on murine normal splenocyte and tumor cells. (a): normal murine splenocyte cell line, (b): B16BL6 tumor cell line, (c): colon26-M3.1 tumor cell line.

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