J Vet Sci.  2016 Sep;17(3):399-405. 10.4142/jvs.2016.17.3.399.

Hematologic and serum biochemical reference intervals of the Oriental white stork (Ciconia boyciana) and the application of an automatic hematologic analyzer

• Han JI
• Jang HJ
• Na KJ

• KMID: 2413141
• DOI: http://doi.org/10.4142/jvs.2016.17.3.399

Abstract

This study was conducted to establish accurate baseline values of clinical laboratory data with regard to age-related changes in the Oriental white stork (Ciconia boyciana). In addition, the availability of an automated hematological cell counter was evaluated. A total of 94 clinically normal storks, including 64 young storks (<1 year old; 30 male and 34 female) and 30 adults (> 1 year old; 17 male and 13 female) were included. Hematological assays were performed using manual and automated cell counters and serum biochemistry profiles were examined using an automated analyzer. There were no significant differences in any parameters between male and female storks, while 16 parameters were significantly different between young and adult storks. Of these 16 parameters, total protein, albumin, aspartate aminotransferase, alanine aminotransferase, creatinine, triglyceride, total bilirubin, potassium, white blood cell count, packed cell volume, mean cell volume and hemoglobin levels were higher in adult storks than in young storks, while the latter showed higher glucose, uric acid and alkaline phosphatase levels, as well as a higher sodium/potassium ratio. The results presented herein will aid researchers who work for the conservation and rehabilitation of this endangered species.

Keyword

Ciconia boyciana; hematology; reference interval; serum biochemistry; stork

MeSH Terms

Age Factors
Animals
Birds/*blood
Blood Chemical Analysis/*veterinary
Cell Count/veterinary
*Conservation of Natural Resources
*Endangered Species
Female
Male
Reference Values
Sex Factors

Figure

• 

  Fig. 1 Passing-Bablok scattergrams showing the agreement between hematological data obtained on manual count and using the Cell-Dyn 3700 for hematological parameters in the Oriental white stork (Ciconia boyciana). The solid red line represents the regression line, the solid gray lines represent the 95% confidence intervals, and the dotted line represents the identity line.

• 

  Fig. 2 Bland-Altman plots showing the agreement between the hematological data obtained on manual count and Cell-Dyn 3700 for hematological parameters in the Oriental white stork (Ciconia boyciana). X-axes represent the average for both methods, and Y-axes represent the difference between assessments.

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