Korean J Parasitol.  2018 Apr;56(2):105-112. 10.3347/kjp.2018.56.2.105.

Atorvastatin: In-Vivo Synergy with Metronidazole as Anti-Blastocystis Therapy

• Basyoni MM
• Fouad SA
• Amer MF
• Amer AF
• Ismail DI

• KMID: 2412728
• DOI: http://doi.org/10.3347/kjp.2018.56.2.105

Abstract

Blastocystis is an enteric Straminopile in tropical, subtropical and developing countries. Metronidazole has been a chemotheraputic for blastocystosis. Failures in its regimens were reported and necessitate new studies searching for alternative therapeutic agents. Aim of current study is to investigate potential effects of Atorvastatin (AVA) compared to the conventional chemotherapeutic MTZ in experimentally Blastocystis-infected mice. Anti-Blastocystis efficacy of AVA was evaluated parasitologically, histopathologically and by transmission electron microscopy using MTZ (10 mg/kg) as a control. Therapeutic efficacy of AVA was apparently dose-dependent. Regimens of AVA (20 and 40 mg/kg) proved effective against Blastocystis infections with high reduction in Blastocystis shedding (93.4-97.9%) compared to MTZ (79.3%). The highest reductions (98.1% and 99.4%) were recorded in groups of combination treatments AVA 20-40 mg/kg and MTZ 10 mg/kg. Blastocystis was nearly eradicated by the 20th day post infection. Genotype analysis revealed that genotype I was most susceptible, genotype III was less. Histopathologic and ultrastructural studies revealed apoptotic changes in Blastocystis and significant improvement of intestinal histopathological changes more remarkable in combinational therapy groups. Thus, the present study offers AVA as a potential candidate for Blastocystis therapy combined with MTZ.

Keyword

Blastocystis; atorvastatin; metronidazole; efficacy; mouse

MeSH Terms

Animals
Atorvastatin Calcium*
Blastocystis
Blastocystis Infections
Developing Countries
Genotype
Metronidazole*
Mice
Microscopy, Electron, Transmission
Atorvastatin Calcium
Metronidazole