J Vet Sci.  2017 Mar;18(1):95-99. 10.4142/jvs.2017.18.1.95.

Probiotic properties and adsorption of Enterococcus faecalis PSCT3-7 to vermiculite

• Kim JY
• Awji EG
• Park NH
• Park JY
• Kim JC
• Lee SP
• Suh JW
• Park SC

• KMID: 2412593
• DOI: http://doi.org/10.4142/jvs.2017.18.1.95

Abstract

The probiotic properties of Enterococcus (E.) faecalis PSCT3-7, a new strain isolated from the intestines of pigs fed dietary fiber containing 50% sawdust, were investigated. E. faecalis PSCT3-7 tolerated a pH range of 3 to 8 and 0.3% bile salts, and it inhibited the growth of Salmonella Typhimurium in a concentration-dependent manner. In addition, E. faecalis showed resistance to several antibacterial agents. Vermiculite, a nutrient and microbial carrier, increased the bile tolerance of the strain. Scanning electron microscope images revealed good adsorption of E. faecalis PSCT3-7 onto vermiculite. E. faecalis PSCT3-7 represents a potential probiotic candidate to administer with vermiculite to swine.

Keyword

Enterococcus faecalis; Salmonella Typhimurium; lactic acid bacteria; probiotics; swine

MeSH Terms

Adsorption
Aluminum Silicates/*chemistry
Animal Feed/analysis
Anti-Bacterial Agents/pharmacology
Diet/veterinary
Enterococcus faecalis/chemistry/drug effects/genetics/*physiology
Phylogeny
Probiotics/administration & dosage/*chemistry/*pharmacology
RNA, Bacterial/genetics
RNA, Ribosomal, 16S/genetics
Aluminum Silicates
Anti-Bacterial Agents
RNA, Bacterial
RNA, Ribosomal, 16S

Figure

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  Fig. 1 Phylogenetic tree showing sequence-based identification of Enterococcus faecalis PSCT3-7.

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  Fig. 2 Growth characteristics of Enterococcus (E.) faecalis PSCT3-7. Changes in pH (A), dissolved oxygen (DO) (B), optical density at 600 nm (OD600) (C), and colony-forming unit per milliliter (CFU/mL) (D) during 48 h fermentation of E. faecalis PSCT3-7. Growth of E. faecalis PSCT3-7 (E) and vermiculite-adsorbed E. faecalis PSCT3-7 (F) in pH-adjusted broth for 2 h at 37℃. The experiments were performed in triplicate (n = 3).

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  Fig. 3 Inhibitory activity of Enterococcus (E.) faecalis PSCT3-7 against Salmonella Typhimurium growth. Co-culture of E. faecalis PSCT3-7 with Salmonella Typhimurium at initial inoculum of 103 CFU/mL (A) and 106 CFU/mL (B) showing E. faecalis PSCT3-7 concentration-dependent inhibition of Salmonella Typhimurium growth. (C and D) The minimum level of E. faecalis PSCT3-7 required to inhibit growth of Salmonella Typhimurium by 50% (IC50) when Salmonella Typhimurium was inoculated at 103 CFU/mL (C) and 106 CFU/mL (D) initial levels. The experiments were performed in triplicate (n = 3). *p < 0.05 compared to the results obtained with control.

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  Fig. 4 Scanning electron microscope images of (A) Enterococcus (E.) faecalis PSCT3-7, (B) vermiculite, and (C) vermiculite-adsorbed E. faecalis PSCT3-7. Scale bar = 1 µm (A), 30 µm (B), 5 µm (C).

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