Mycobiology.  2008 Jun;36(2):102-105.

Production of the Acetylcholinesterase Inhibitor from Yarrowia lipolytica S-3

• Lee DH
• Lee JS
• Yi SH
• Lee JS

Abstract

The acetylcholinesterase (AChE) inhibitor of Yarrowia lipolytica S-3 was maximally produced when it was incubated at 30degrees C for 36 h in an optimal medium containing 1% yeast extract, 2% peptone and 2% glucose, with an initial pH 6.0. The final AChE inhibitory activity under these conditions was an IC50 value of 64 mg/ml. After partial purification of the AChE inhibitor by means of systematic solvent extraction, the final IC50 value of the partially purified AChE inhibitor was 0.75 mg/ml. We prepared a test product by using the partially purified AChE inhibitor and then determined its stability for the development of a new antidementia commercial product. The test product was stable at room temperature for 15 weeks.

Keyword

Acetylcholinesterase inhibitor; Antidementia; Yarrowia lipolytica S-3

MeSH Terms

Acetylcholinesterase
Glucose
Hydrogen-Ion Concentration
Inhibitory Concentration 50
Peptones
Yarrowia
Yeasts
Acetylcholinesterase
Glucose
Peptones