Tissue Eng Regen Med.  2018 Feb;15(1):1-11. 10.1007/s13770-017-0082-x.

Oral Immunization of FMDV Vaccine Using pH-Sensitive and Mucoadhesive Thiolated Cellulose Acetate Phthalate Microparticles

• Lee HB
• Yoon SY
• Singh B
• Oh SH
• Cui L
• Yan C
• Kang SK
• Choi YJ
• Cho CS

• KMID: 2405963
• DOI: http://doi.org/10.1007/s13770-017-0082-x

Abstract

Several barriers such as gastric pH, enzymatic degradation and rapid transit should be overcome to orally deliver antigens for taking up by epithelial microfold cells in Peyer's patches of small intestine. To solve the above mentioned problems, we designed pH-sensitive and mucoadhesive polymeric microparticles (MPs) prepared by double emulsion technique using cellulose acetate phthalate (CAP) to enhance immune response of foot-and-mouth disease (FMD) virus (FMDV) subunit vaccine. Thiolation of CAP improved mucoadhesive property of CAP to prolong the MPs transit time through the gastrointestinal tract. Thiolated CAP (T-CAP) also slowed down antigen release in acidic pH of stomach but released more antigens in neutral pH of small intestine due to the pH-sensitivity of the T-CAP. Oral immunization of a chimerical multi-epitope recombinant protein as the FMD subunit vaccine via T-CAP MPs effectively delivered the vaccine to Peyer's patches eliciting mucosal IgA response. It will make a step forward into a promising oral subunit vaccine development in livestock industry.

Keyword

pH-senstive; Mucoadhesive; Foot-and-mouth disease; Subunit vaccine; Oral immunization

MeSH Terms

Animals
Cellulose*
Foot-and-Mouth Disease
Gastrointestinal Tract
Hydrogen-Ion Concentration
Immunization*
Immunoglobulin A
Intestine, Small
Livestock
Peyer's Patches
Polymers
Staphylococcal Protein A
Stomach
Cellulose
Immunoglobulin A
Polymers
Staphylococcal Protein A