Restor Dent Endod.  2017 Nov;42(4):316-323. 10.5395/rde.2017.42.4.316.

Comparison of apical extrusion of intracanal bacteria by various glide-path establishing systems: an in vitro study

• Dagna A
• El Abed R
• Hussain S
• Abu-Tahun
• Visai L
• Bertoglio
• Bosco F
• Beltrami R
• Poggio C
• Kim HC

• KMID: 2403073
• DOI: http://doi.org/10.5395/rde.2017.42.4.316

Abstract

OBJECTIVES
This study compared the amount of apically extruded bacteria during the glide-path preparation by using multi-file and single-file glide-path establishing nickel-titanium (NiTi) rotary systems.
MATERIALS AND METHODS
Sixty mandibular first molar teeth were used to prepare the test apparatus. They were decoronated, blocked into glass vials, sterilized in ethylene oxide gas, infected with a pure culture of Enterococcus faecalis, randomly assigned to 5 experimental groups, and then prepared using manual stainless-steel files (group KF) and glide-path establishing NiTi rotary files (group PF with PathFiles, group GF with G-Files, group PG with ProGlider, and group OG with One G). At the end of canal preparation, 0.01 mL NaCl solution was taken from the experimental vials. The suspension was plated on brain heart infusion agar and colonies of bacteria were counted, and the results were given as number of colony-forming units (CFU).
RESULTS
The manual instrumentation technique tested in group KF extruded the highest number of bacteria compared to the other 4 groups (p < 0.05). The 4 groups using rotary glide-path establishing instruments extruded similar amounts of bacteria.
CONCLUSIONS
All glide-path establishment instrument systems tested caused a measurable apical extrusion of bacteria. The manual glide-path preparation showed the highest number of bacteria extruded compared to the other NiTi glide-path establishing instruments.

Keyword

Bacterial extrusion; Debris extrusion; Glide-path; Manual instrumentation; Nickel-titanium rotary file

MeSH Terms

Agar
Bacteria*
Brain
Enterococcus faecalis
Ethylene Oxide
Glass
Heart
In Vitro Techniques*
Molar
Stem Cells
Tooth
Agar
Ethylene Oxide

Figure

• 

  Figure 1 The schematic diagram of the methods used in this study. WL, working length; E/O, ethylene oxide; CFU, colony-forming unit.

• 

  Figure 2 The manual instrumentation technique tested in K-file extruded the highest number of bacteria compared to the other groups. CFU, colony-forming unit. *It means significant differences from other groups, p < 0.05.

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