Korean J Otorhinolaryngol-Head Neck Surg.  2017 Nov;60(11):570-574. 10.3342/kjorl-hns.2017.00451.

Comparison of Methods for Collecting Saliva for Pepsin Detection in Patients with Laryngopharyngeal Reflux

• Ryu IY
• Jung AR
• Park JM
• Lee YC
• Eun YG

• KMID: 2396864
• DOI: http://doi.org/10.3342/kjorl-hns.2017.00451

Abstract

BACKGROUND AND OBJECTIVES
To compare the simple spitting method and the Salivette® method of collecting saliva for detecting pepsin in patients with laryngopharyngeal reflux disease (LPRD).
SUBJECTS AND METHOD
Thirty-two patients diagnosed with LPRD by 24 hour multichannel intraluminal impedance and pH monitoring were enrolled prospectively. The amounts of pepsin in saliva determined by the simple spitting method and the Salivette® method were compared.
RESULTS
Simple spitting showed higher sensitivity, specificity, accuracy, positive predictive value and negative predictive value. There was no statistically significant difference between the amount of pepsin detected by simple spitting (10.07±11.68 ng/mL) versus that detected using the Salivette® method (7.09±7.27 ng/mL) (p=0.258).
CONCLUSIONS
The simple spitting method has higher sensitivity, specificity and accuracy than the Salivette® method for detecting pepsin in patients with LPRD.

Keyword

Laryngopharyngeal reflux; Pepsin; Saliva; Salivette

MeSH Terms

Electric Impedance
Humans
Hydrogen-Ion Concentration
Laryngopharyngeal Reflux*
Methods*
Pepsin A*
Prospective Studies
Saliva*
Sensitivity and Specificity
Pepsin A