Lab Anim Res.  2011 Mar;27(1):47-52. 10.5625/lar.2011.27.1.47.

Different Antiulcer Activities of Pantoprazole in Stress, Alcohol and Pylorus Ligation-Induced Ulcer Models

• Bae DK
• Park D
• Lee SH
• Yang G
• Yang YH
• Kim TK
• Choi YJ
• Kim JJ
• Jeon JH
• Jang MJ
• Choi EK
• Hwang SY
• Kim YB

• KMID: 2391858
• DOI: http://doi.org/10.5625/lar.2011.27.1.47

Abstract

Antiulcer effects of pantoprazole, a proton-pump inhibitor, on water-immersion restraint stress (WIRS)-, alcohol (ethanol)- and pylorus ligation-induced gastric ulcers were investigated in male rats. Rats were orally administered with pantoprazole 30 min prior to exposure to various types of ulcer inducers. In stress-induced ulcer model, rats were subjected to WIRS at 22degrees C for 4 hours, and the degree of ulcer (in mm) was evaluated. In alcohol-induced ulcer model, rats were orally administered with pure (100%) ethanol (1 mL/kg), and the ulcer lesions were measured 1 hour after ethanol challenge. In pylorus ligation-induced ulcer model, rats were subjected to pylorus ligation, and the degree of erosions and ulcers was scored 17 hours after the operation. Pantoprazole attenuated the ulcer lesions induced by WIRS in a dose-dependent manner, exhibiting a median effective dose (ED50) value of 0.78 mg/kg. By comparison, pantoprazole was effective at relatively-high doses for the improvement of ethanol-induced ulcers, showing an ED50 value of 20.5 mg/kg. Notably, pantoprazole was practically ineffective (ED50>50.0) in pylorus ligation model. Taken together, it was confirmed that pantoprazole showed inhibitory activity on gastric ulcers induced by stress and alcohol, but was ineffective on pylorus ligation-induced ulcer. Therefore, the results indicate that proton-pump inhibitors including pantoprazole might reveal highly-different effects according to the type of ulcer inducers, and that the prescription of antiulcer agents should be carefully selected.

Keyword

Pantoprazole; gastric ulcer; stress; alcohol; pylorus ligation

MeSH Terms

2-Pyridinylmethylsulfinylbenzimidazoles
Animals
Ethanol
Humans
Ligation
Male
Prescriptions
Pylorus
Rats
Stomach Ulcer
Ulcer
2-Pyridinylmethylsulfinylbenzimidazoles
Ethanol

Figure

• 

  Figure 1 Representative findings of gastric ulcers (arrows) induced by water-immersion restraint stress. Rats were placed in a restraint device, immersed up to their xiphoid process in a 22℃ water bath, and sacrificed 4 hours later for the measurement of ulcer lesions. A, vehicle control; B, 5 mg/kg pantoprazole. Scale bar=1 mm.

• 

  Figure 2 Representative findings of gastric ulcers (arrows) induced by ethanol (1 mL/kg). Rats were orally administered with 1 mL/kg of pure ethanol, and sacrificed 1 hour later for the measurement of ulcer lesions. A, vehicle control; B, 10 mg/kg pantoprazole. Scale bar=1 mm.

• 

  Figure 3 Representative findings of gastric ulcers (arrows) induced by pylorus ligation. Rats were subjected to an operation of pylorus ligation, and sacrificed 17 hours later for scoring of ulcer lesions. A, vehicle control; B, 25 mg/kg pantoprazole.

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