J Periodontal Implant Sci.  2016 Feb;46(1):35-45. 10.5051/jpis.2016.46.1.35.

Prevalence of Porphyromonas gingivalis fimA genotypes in the peri-implant sulcus of Koreans assessed using a new primer

• Kim SG
• Hong JY
• Shin SI
• Moon JH
• Lee JY
• Herr Y

• KMID: 2391728
• DOI: http://doi.org/10.5051/jpis.2016.46.1.35

Abstract

PURPOSE
Porphyromonas gingivalis fimA is a virulence factor associated with periodontal diseases, but its role in the pathogenesis of peri-implantitis remains unclear. We aimed to evaluate the relationship between the condition of peri-implant tissue and the distribution of P. gingivalis fimA genotypes in Koreans using a new primer.
METHODS
A total of 248 plaque samples were taken from the peri-implant sulci of 184 subjects. The control group consisted of sound implants with a peri-implant probing depth (PD) of 5 mm or less with no bleeding on probing (BOP). Test group I consisted of implants with a peri-implant PD of 5 mm or less and BOP, and test group II consisted of implants with a peri-implant PD of more than 5 mm and BOP. DNA was extracted from each sample and analyzed a using a polymerase chain reaction (PCR) with P. gingivalis-specific primers, followed by an additional PCR assay to differentiate the fimA genotypes in P. gingivalis- positive subjects.
RESULTS
The Prevalence of P. gingivalis in each group did not significantly differ (P>0.05). The most predominant fimA genotype in all groups was type II. The prevalence of type Ib fimA was significantly greater in test group II than in the control group (P<0.05).
CONCLUSIONS
The fimA type Ib genotype of P. gingivalis was found to play a critical role in the destruction of peri-implant tissue, suggesting that it may be a distinct risk factor for peri-implantitis.

Keyword

Fimbriae; Peri-implantitis; Periodontal diseases; Virulence factors

MeSH Terms

DNA
Genotype*
Hemorrhage
Peri-Implantitis
Periodontal Diseases
Polymerase Chain Reaction
Porphyromonas gingivalis*
Porphyromonas*
Prevalence*
Risk Factors
Virulence
Virulence Factors
DNA
Virulence Factors

Figure

• 

  Figure 1 Electrophoresis of amplification products on a 1.8% agarose gel. Lines 1–6 are type I, type Ib, type II (new), type III, type IV, and type V. M, molecular weight marker.

• 

  Figure 2 Detection of type Ib fimA by polymerase chain reaction amplification and RsaI digestion. Lanes 1–3 comprise fimA amplicons from a mixed culture of P. gingivalis strains (ATCC33277 for type I fimA and HG1691for type Ib fimA), the pure culture of strain ATCC33277, and the pure culture of strain HG1691, respectively, using type Ib primers. Lanes 4–6 show the amplicons of lanes 1-3 digested with RsaI. M, molecular weight marker.

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