Lab Anim Res.  2017 Sep;33(3):280-282. 10.5625/lar.2017.33.3.280.

Super pregnancy in a BALB/c mouse superovulated with PMSG

• Vaseghi H
• Mogheiseh
• Sepehrimanesh M
• Kafi M
• Nooranizadeh MH

• KMID: 2391103
• DOI: http://doi.org/10.5625/lar.2017.33.3.280

Abstract

This paper reports a case of super pregnancy in a BALB/c mouse pregnant with 30 pups following induction of superovulation using a PMSG-hCG protocol. Superovulation was induced in 10 mice by injecting 5 IU PMSG followed by 5 IU hCG 48 hours later. Immediately after injection of hCG, animals were placed with males at a ratio of 1 to 1 for 24 hours. On day 14 after mating, animals were killed by cervical dislocation and the uterus was examined for pregnancy and the number of fetuses. The mean (±SEM) number of fetuses observed in ten mice was 5.4±3.18 with an unexpectedly super pregnant mouse bearing 30 fetuses on day 14 of pregnancy.

Keyword

Super pregnancy; superovulation; mice; PMSG

MeSH Terms

Animals
Dislocations
Fetus
Humans
Male
Mice*
Pregnancy*
Superovulation
Uterus

Figure

• 

  Figure 1 A mouse Superovulated with PMSG (5IU) and hCG (5IU) and it's thirty fetal sac 14 days postmating.

• 

  Figure 2 Thirty 14-days old fetuses of mouse Superovulated with PMSG and hCG.

• 

  Figure 3 Ovary with corpora lutea and some follicles from amouce Superovulated with PMSG and hCG (×10).

Reference

1. Rugh R. The mouse. Its reproduction and development. Burgess Pub. Co.;1968. p. 225–235.

2. Luo C, Zuñiga J, Edison E, Palla S, Dong W, Parker-Thornburg J. Superovulation strategies for 6 commonly used mouse strains. J Am Assoc Lab Anim Sci. 2011; 50(4):471–478.

3. EDWARDS RG, FOWLER RE. Fetal mortality in adult mice after superovulation with gonadotrophins. J Exp Zool. 1959; 141:299–322.

4. Van der Auwera I, D'Hooghe T. Superovulation of female mice delays embryonic and fetal development. Hum Reprod. 2001; 16(6):1237–1243.

5. Sato A. Some observations on induced polyovulation and superpregnancy in mature mice. Embryologia. 1963; 7:285–294.

6. Hasegawa A, Mochida K, Inoue H, Noda Y, Endo T, Watanabe G, Ogura A. High-Yield Superovulation in Adult Mice by Anti-Inhibin Serum Treatment Combined with Estrous Cycle Synchronization. Biol Reprod. 2016; 94(1):21.

7. Corbin TJ, McCabe JG. Strain variation of immature female rats in response to various superovulatory hormone preparations and routes of administration. Contemp Top Lab Anim Sci. 2002; 41(2):18–23.

8. Behringer R, Gertsenstein M, Nagy K, Nagy A. Manipulating the mouse embryo: a laboratory manual. Cold Spring Harbor Laboratory Press;2013. p. 345–360.

9. Johnson AD. Limitation of fetus number in the rat, mouse and rabbit. J Anim Sci. 1970; 30(6):978–983.

10. Ohta H, Sakaide Y, Wakayama T. Age- and substrain-dependent sperm abnormalities in BALB/c mice and functional assessment of abnormal sperm by ICSI. Hum Reprod. 2009; 24(4):775–781.

11. Ozgunen KT, Erdogan S, Mazmanoglu N, Pamuk I, Logoglu G, Ozgunen T. Effect of gonadotrophin dose on oocyte retrieval in superovulated BALB/c mice. Theriogenology. 2001; 56(3):435–445.

12. Mauleon P. Oogenesis and Folliculogenesis. Reproduction in Domestic Animals. Second Edition. Academic Press;1969. p. 187–215.

13. Pontes JH, Melo Sterza FA, Basso AC, Ferreira CR, Sanches BV, Rubin KC, Seneda MM. Ovum pick up, in vitro embryo production, and pregnancy rates from a large-scale commercial program using Nelore cattle (Bos indicus) donors. Theriogenology. 2011; 75(9):1640–1646.

14. Loutradis D, Drakakis P, Vomvolaki E, Antsaklis A. Different ovarian stimulation protocols for women with diminished ovarian reserve. J Assist Reprod Genet. 2007; 24(12):597–611.

15. Lubritz DL, Eisen EJ, Robison OW. Effect of selection for litter size and body weight on hormone-induced ovulation rate in mice. J Anim Sci. 1991; 69(11):4299–4305.

16. Spearow JL, Barkley M. Genetic control of hormone-induced ovulation rate in mice. Biol Reprod. 1999; 61(4):851–856.

17. Argente MJ, Santacreu MA, Climent A, Blasco A. Influence of available uterine space per fetus on fetal development and prenatal survival in rabbits selected for uterine capacity. Livestock Science. 2006; 102:83–91.