Clin Nutr Res.  2017 Jul;6(3):198-205. 10.7762/cnr.2017.6.3.198.

Effects of Vitamin C Supplementation on Plasma and Urinary Vitamin C Concentration in Korean Women

• Choi J
• Kim DY
• Choue R
• Lim H

• KMID: 2385624
• DOI: http://doi.org/10.7762/cnr.2017.6.3.198

Abstract

Although vitamin C supplements were consumed for health maintenance and fatigue recovery, the effects of high doses of vitamin C supplement remains controversial. Our study performed the effects of 100 mg and 2,000 mg vitamin C supplements on plasma and urinary vitamin C concentration in Korean women. Twenty-four women completed the 4 weeks intervention. Anthropometric data, plasma and urinary vitamin C concentrations, superoxide dismutase activity, thiobarbituric acid reactive substance (TBARS) level, and fatigue severity scale (FSS) were collected, and the statistical analyses compared between- and within-group findings at pre- and post-intervention. Concentrations of vitamin C in plasma and urinary excretion were significantly increased with 100 mg and 2,000 mg of vitamin C supplementation (p < 0.050). TBARS level was decreased significantly with 2,000 mg of vitamin C supplementation (p < 0.050). In addition, FSS was declined significantly in 100 mg of vitamin C supplementation group (p < 0.050). Our result showed that vitamin C supplementation of either 100 mg or 2,000 mg led to an increase in vitamin C concentrations in plasma and vitamin urinary excretion but not statistically significant among groups. TBARS level was decreased in 2,000 mg and FSS was decreased in 100 mg of vitamin C supplementation in Korean women. We suppose that additional clinical trial is needed to examine the effects of vitamin C supplements for a wide range of doses on plasma and urinary vitamin C concentrations in Korean.

Keyword

Vitamin C supplement; Plasma vitamin C concentration; Urinary vitamin C excretion; Oxidative stress biomarkers; Fatigue

MeSH Terms

Ascorbic Acid*
Fatigue
Female
Humans
Plasma*
Superoxide Dismutase
Thiobarbituric Acid Reactive Substances
Vitamins*
Ascorbic Acid
Superoxide Dismutase
Thiobarbituric Acid Reactive Substances
Vitamins

Reference

1. Omaye ST, Skala JH, Jacob RA. Plasma ascorbic acid in adult males: effects of depletion and supplementation. Am J Clin Nutr. 1986; 44:257–264. PMID: 3728363.

2. Levine M, Conry-Cantilena C, Wang Y, Welch RW, Washko PW, Dhariwal KR, Park JB, Lazarev A, Graumlich JF, King J, Cantilena LR. Vitamin C pharmacokinetics in healthy volunteers: evidence for a recommended dietary allowance. Proc Natl Acad Sci USA. 1996; 93:3704–3709. PMID: 8623000.

3. Alessio HM, Goldfarb AH, Cao G. Exercise-induced oxidative stress before and after vitamin C supplementation. Int J Sport Nutr. 1997; 7:1–9. PMID: 9063760.

4. Ministry of Health and Welfare, Korea Centers for Disease Control and Prevention. Korea Health Statistics 2015: Korea National Health and Nutrition Examination Survey (KNHANES VI-3). Sejong: Korea Centers for Disease Control and Prevention;2016.

5. Ministry of Health and Welfare (KR). The Korean Nutrition Society. Dietary reference intakes for Koreans 2015. Sejong: Ministry of Health and Welfare;2016.

6. Levine M, Wang Y, Padayatty SJ, Morrow J. A new recommended dietary allowance of vitamin C for healthy young women. Proc Natl Acad Sci USA. 2001; 98:9842–9846. PMID: 11504949.

7. Krupp LB, LaRocca NG, Muir-Nash J, Steinberg AD. The fatigue severity scale. Application to patients with multiple sclerosis and systemic lupus erythematosus. Arch Neurol. 1989; 46:1121–1123. PMID: 2803071.

8. Melethil S, Mason WD, Chian-Jo C. Dose-dependent absorption and excretion of vitamin C in humans. Int J Pharm. 1986; 31:83–89.

9. Lee SS, Choi IS, Lee KW, Oh SH. Effect of antioxidant vitamin supplementation on blood composition in smoking college men. J Nutr Health. 1998; 31:289–296.

10. Baker EM, Hodges RE, Hood J, Sauberlich HE, March SC, Canham JE. Metabolism of 14C- and 3H-labeled L-ascorbic acid in human scurvy. Am J Clin Nutr. 1971; 24:444–454. PMID: 5090632.

11. Machlin LJ, Bendich A. Free radical tissue damage: protective role of antioxidant nutrients. FASEB J. 1987; 1:441–445. PMID: 3315807.

12. Lim JY, Kim JH. The effects of vitamin C supplementation and nutrition education on nutrition knowledge, food habits and antioxidative enzyme activity in male adolescent smokers. Korean J Community Nutr. 2001; 6:282–289.

13. Gultekin F, Ozturk M, Akdogan M. The effect of organophosphate insecticide chlorpyrifos-ethyl on lipid peroxidation and antioxidant enzymes (in vitro). Arch Toxicol. 2000; 74:533–538. PMID: 11131033.

14. Janero DR. Malondialdehyde and thiobarbituric acid-reactivity as diagnostic indices of lipid peroxidation and peroxidative tissue injury. Free Radic Biol Med. 1990; 9:515–540. PMID: 2079232.

15. Yoon MY, Lee JW. Effect of excess vitamin C on serum lipid peroxide value in young and elderly men. Chungnam J Hum Ecol. 1988; 1:81–91.

16. Prince MI, Mitchison HC, Ashley D, Burke DA, Edwards N, Bramble MG, James OF, Jones DE. Oral antioxidant supplementation for fatigue associated with primary biliary cirrhosis: results of a multicentre, randomized, placebo-controlled, cross-over trial. Aliment Pharmacol Ther. 2003; 17:137–143. PMID: 12492743.

17. Kodama M, Kodama T, Murakami M. The value of the dehydroepiandrosterone-annexed vitamin C infusion treatment in the clinical control of chronic fatigue syndrome (CFS). II. Characterization of CFS patients with special reference to their response to a new vitamin C infusion treatment. In Vivo. 1996; 10:585–596. PMID: 8986468.

18. Hodges RE, Baker EM, Hood J, Sauberlich HE, March SC. Experimental scurvy in man. Am J Clin Nutr. 1969; 22:535–548. PMID: 4977512.