Korean J Parasitol.  2015 Jun;53(3):335-339. 10.3347/kjp.2015.53.3.335.

Cloning and Iron Transportation of Nucleotide Binding Domain of Cryptosporidium andersoni ATP-Binding Cassette (CaABC) Gene

• Wang JH
• Xue XH
• Zhou J
• Fan CY
• Xie QQ
• Wang P

• KMID: 2381838
• DOI: http://doi.org/10.3347/kjp.2015.53.3.335

Abstract

Cryptosporidium andersoni ATP-binding cassette (CaABC) is an important membrane protein involved in substrate transport across the membrane. In this research, the nucleotide binding domain (NBD) of CaABC gene was amplified by PCR, and the eukaryotic expression vector of pEGFP-C1-CaNBD was reconstructed. Then, the recombinant plasmid of pEGFP-C1-CaNBD was transformed into the mouse intestinal epithelial cells (IECs) to study the iron transportation function of CaABC. The results indicated that NBD region of CaABC gene can significantly elevate the transport efficiency of Ca2+, Mg2+, K+, and HCO3 - in IECs (P<0.05). The significance of this study is to find the ATPase inhibitors for NBD region of CaABC gene and to inhibit ATP binding and nutrient transport of CaABC transporter. Thus, C. andersoni will be killed by inhibition of nutrient uptake. This will open up a new way for treatment of cryptosporidiosis.

Keyword

Cryptosporidium andersoni; cloning; iron transportation; ATP-binding cassette transporter protein

MeSH Terms

ATP-Binding Cassette Transporters/*chemistry/*genetics/metabolism
Adenosine Triphosphate/metabolism
Amino Acid Sequence
Animals
Calcium/metabolism
*Cloning, Molecular
Cryptosporidiosis/parasitology
Cryptosporidium/chemistry/genetics/*metabolism
Humans
Iron/metabolism
Mice
Molecular Sequence Data
Protein Structure, Tertiary
Protozoan Proteins/*chemistry/*genetics/metabolism
Sequence Alignment
Adenosine Triphosphate
Calcium
Iron
Protozoan Proteins