Ann Dermatol.  2017 Jun;29(3):367-370. 10.5021/ad.2017.29.3.367.

Attenuated Nuclear Factor Kappa B Activity by E7 Protein of Human Papillomavirus Type 2 in Human Epidermal Keratinocytes

• Ryu YH
• Han JH
• Lee JH
• Choi SY
• Park YM

• KMID: 2378546
• DOI: http://doi.org/10.5021/ad.2017.29.3.367

Abstract

No abstract available.

MeSH Terms

Humans*
Keratinocytes*
NF-kappa B*
NF-kappa B

Figure

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  Fig. 1 Human papillomavirus type 2 (HPV2) E7 (A, B) and toll-like receptor 9 (TLR9) (C, D) protein expression in normal human skin and wart lesions. TLR9 mRNA expression in wart lesions (E) and normal human keratinocytes (NHKs) transfected with HPV2 E7 (F). TLR9 protein and mRNA expressions were assessed by immunohistochemical staining and quantitative real time polymerase chain reaction, respectively. The specific antibodies for HPV2 E7 and TLR9 were diluted with phosphate-buffered saline (1:100). Original magnification: ×200. TLR9 specific primer was used and normalized to 18s rRNA in the quantitative real time polymerase chain reaction. Each bar indicates the mean±standard error. *p<0.05. NS: normal human skin, RFI: relative fold increase, pCMV-Δ: NHKs transfected with empty vector, pCMV::E7: NHKs transfected with HPV2 E7.

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  Fig. 2 (A) Effect of human papillomavirus type 2 (HPV2) E7 on the nuclear factor kappa B (NF-κB) activity of the normal human keratinocytes (NHKs). NHKs were pretreated with tumor necrosis factor-α (TNF-α) (50 ng/ml) for 3 hours before HPV2 E7 transfection. A luciferase assay using the NF-κB dependent firefly luciferase reporter vector was performed. Values of NF-κB activity were normalized to β-galactosidase activity. Each bar indicates the mean±standard error. *p<0.05. (B) The nuclear translocation of p65 was investigated by immunofluorescence staining. Nuclei were stained with 4, 6-diamidino-2-phenylindole. Original magnification: ×400. pCMV-Δ: NHKs transfected with empty vector, pCMV::E7: NHKs transfected with HPV2 E7, DAPI: 4′,6-diamidino-2-phenylindole.

Reference

1. Stanley MA. Epithelial cell responses to infection with human papillomavirus. Clin Microbiol Rev. 2012; 25:215–222.

2. Zekan J, Skerlev M, Milić L, Karelovć D. Human papillomavirus-related diseases of the female lower genital tract: oncogenic aspects and molecular interaction. Coll Antropol. 2014; 38:779–786.

3. McLaughlin-Drubin ME, Münger K. The human papillomavirus E7 oncoprotein. Virology. 2009; 384:335–344.

4. Oeckinghaus A, Hayden MS, Ghosh S. Crosstalk in NF-κB signaling pathways. Nat Immunol. 2011; 12:695–708.

5. Ku JK, Kwon HJ, Kim MY, Kang H, Song PI, Armstrong CA, et al. Expression of Toll-like receptors in verruca and molluscum contagiosum. J Korean Med Sci. 2008; 23:307–314.

6. Choi Y, Kim DH, Jin SY, Lee AY, Lee SH. Topical immunotherapy with diphenylcyclopropenone is effective and preferred in the treatment of periungual warts. Ann Dermatol. 2013; 25:434–439.

7. Kim SY, Jung SK, Lee SG, Yi SM, Kim JH, Kim IH. New alternative combination therapy for recalcitrant common warts: the efficacy of imiquimod 5% cream and duct tape combination therapy. Ann Dermatol. 2013; 25:261–263.

8. Vandermark ER, Deluca KA, Gardner CR, Marker DF, Schreiner CN, Strickland DA, et al. Human papillomavirus type 16 E6 and E 7 proteins alter NF-kB in cultured cervical epithelial cells and inhibition of NF-kB promotes cell growth and immortalization. Virology. 2012; 425:53–60.

9. Valentine R, Dawson CW, Hu C, Shah KM, Owen TJ, Date KL, et al. Epstein-Barr virus-encoded EBNA1 inhibits the canonical NF-kappaB pathway in carcinoma cells by inhibiting IKK phosphorylation. Mol Cancer. 2010; 9:1.

10. Sloan E, Henriquez R, Kinchington PR, Slobedman B, Abendroth A. Varicella-zoster virus inhibition of the NF-κB pathway during infection of human dendritic cells: role for open reading frame 61 as a modulator of NF-κB activity. J Virol. 2012; 86:1193–1202.