Yeungnam Univ J Med.  1991 Jun;8(1):53-62. 10.12701/yujm.1991.8.1.53.

Antibacterial effects of immunoglobulin alone and in combination with ciprofloxacin against pseudomonas aeruginosa

• Sung YO
• Kim HS
• Jeon TI
• Kim SK

• KMID: 2377511
• DOI: http://doi.org/10.12701/yujm.1991.8.1.53

Abstract

Experiments were performed in mice (Balb/C) to support the basic efficacy of the human immunoglobulin (IgG) preparation. The antibacterial activity of IgG purified from human sera was examined with or without the quinolone agent, ciprofloxacin (CPFX), against Pseudomonas aeruginosa isolated from clinical specimens.
Results
were as follows: Antibacterial activities in terms of percentage of survivors, after administration of Ps. aeruginosa into mouse intraperitoneal cavity were in the following order, single IgG group, CPFX administration after IgG pretreatment group, IgG and CPFX combined administration group and CPFX alone group. The number of living bacteria was monitored in blood and liver tissue of mice infected with Ps. aeruginosa and treated by IgG administration. The increase of living bacteria in liver was more drastic than that in blood. Leukocytosis was observed in mice injected with IgG, excluding those only with ciprofloxacin, after 8 hours of administration to see a decrease to normal number of bacteria after 18 hours. No significant difference was noticed between pretreatment group and post treatment group. In vitro susceptibility test of IgG against Ps. aeruginosa, minimal inhibitory concentration (MIC) was 250 µg/ml, resistant to IgG, regardless of a combined administration with CPFX. In vitro test revealed that the IgG itself did not have anti-Ps. aeruginosa activity.

MeSH Terms

Animals
Bacteria
Ciprofloxacin*
Humans
Immunoglobulin G
Immunoglobulins*
In Vitro Techniques
Leukocytosis
Liver
Mice
Pseudomonas aeruginosa*
Pseudomonas*
Survivors
Ciprofloxacin
Immunoglobulin G
Immunoglobulins