Infect Chemother.  2017 Mar;49(1):57-61. 10.3947/ic.2017.49.1.57.

Establishment of Experimental Murine Peritonitis Model with Hog Gastric Mucin for Carbapenem-Resistant Gram-Negative Bacteria

Affiliations
  • 1Vaccine Bio Research Institute, College of Medicine, The Catholic University of Korea, Seoul, Korea. symonlee@catholic.ac.kr
  • 2Division of Infectious Diseases, Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea.

Abstract

Animal models are essential to studies of infectious diseases. The use of mice to test bacterial infection has been extensively reported. However, methods applied to clinical isolates, particularly for carbapenem-resistant bacteria, must be tailored according to the infection models and bacteria used. In this study, we infected 6-week-old female BALB/c mice intraperitoneally with different strains of resistant bacteria plus 3% hog gastric mucin. This method was found to be efficient and readily applicable for investigation of carbapenem-resisant Gram-negative pathogens (e.g., Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Acinetobacter baumannii) detected in Korea.

Keyword

Laboratory animal models; Peritonitis; Drug resistance, Multidrug resistance; Gram-negative bacteria

MeSH Terms

Acinetobacter
Animals
Bacteria
Bacterial Infections
Communicable Diseases
Escherichia coli
Female
Gastric Mucins*
Gram-Negative Bacteria*
Humans
Klebsiella pneumoniae
Korea
Methods
Mice
Models, Animal
Peritonitis*
Pseudomonas aeruginosa
Gastric Mucins

Figure

  • Figure 1 Carbapenem-resistant Escherichia coli (EC-1), Klebsiella pneumoniae (KP-4), Pseudomonas aeruginosa (PA-3) and Acinetobacter baumannii (Aci_100087) with or without 3% hog gastric mucin. Results show that animals inoculated with strains without mucin treatment failed in infection for both mice species, whereas with mucin, infection was successfully induced 24 h after the experiment except for E. coli infection of ICR mice. 3% hog gastric mucin dissolved in phosphate-buffered saline was used as a negative control for each experimental group.


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