Mycobiology.  2016 Dec;44(4):277-282. 10.5941/MYCO.2016.44.4.277.

Community Structures of Arbuscular Mycorrhizal Fungi in Soils and Plant Roots Inhabiting Abandoned Mines of Korea

• Park H
• Lee EH
• Ka KH
• Eom AH

• KMID: 2366452
• DOI: http://doi.org/10.5941/MYCO.2016.44.4.277

Abstract

In this study, we collected rhizosphere soils and root samples from a post-mining area and a natural forest area in Jecheon, Korea. We extracted spores of arbuscular mycorrhizal fungi (AMF) from rhizospheres, and then examined the sequences of 18S rDNA genes of the AMF from the collected roots of plants. We compared the AMF communities in the post-mining area and the natural forest area by sequence analysis of the AMF spores from soils and of the AMF clones from roots. Consequently, we confirmed that the structure of AMF communities varied between the post-mining area and the natural forest area and showed significant relationship with heavy metal contents in soils. These results suggest that heavy metal contamination by mining activity significantly affects the AMF community structure.

Keyword

Arbuscular mycorrhizal fungi; Community; Diversity; DNA cloning; Mycorrhizae

MeSH Terms

Chungcheongbuk-do
Clone Cells
DNA, Ribosomal
Forests
Fungi*
Korea*
Mining
Mycorrhizae
Plant Roots*
Plants*
Rhizosphere
Sequence Analysis
Soil*
Spores
DNA, Ribosomal
Soil