Immune Netw.  2016 Oct;16(5):311-315. 10.4110/in.2016.16.5.311.

Genetic Characteristics and Immunogenicity of Pandemic H1N1 Influenza Virus Isolate from Pig in Korea

• Moon HJ
• Oh JS
• Na W
• Yeom M
• Han SY
• Kim SJ
• Park BK
• Song DS
• Kang BK

• KMID: 2355025
• DOI: http://doi.org/10.4110/in.2016.16.5.311

Abstract

A pandemic influenza A (H1N1) virus strain was isolated from a pig farm in Korea in December 2009. The strain was propagated in and isolated from both the Madin-Darby canine kidney cell line and embryonated eggs. The partial and complete sequences of the strain were identical to those of A/California/04/2009, with >99% sequence similarity in the HA, NA, M, NS, NP, PA, PB1, and PB2 genes. The isolated strain was inactivated and used to prepare a swine influenza vaccine. This trial vaccine, containing the new isolate that has high sequence similarity with the pandemic influenza A (H1N1) virus, resulted in seroconversion in Guinea pigs and piglets. This strain could therefore be a potential vaccine candidate for swine influenza control in commercial farms.

Keyword

Influenza; Pig; Vaccine; H1N1

MeSH Terms

Agriculture
Animals
Cell Line
Eggs
Guinea Pigs
Influenza Vaccines
Influenza, Human*
Kidney
Korea*
Orthomyxoviridae*
Ovum
Pandemics*
Seroconversion
Swine
Influenza Vaccines

Figure

• 

  Figure 1 Phylogenetic analysis of the HA (A) and NA (B) gene sequences of A/swine/Korea/GCVP-KS01/2010 (H1N1). The H1N1 reference strains are as follows: A/Gangwon/1805/2009 (H1N1): CY060460/CY060461; A/California/04/2009 (H1N1): GQ280797/FJ969517; A/Texas/44312415/2009 (H1N1): CY052338/CY052340; A/Singapore/ON355/2009 (H1N1): CY049372/CY049374; A/Australia/4/2009 (H1N1): CY055067/CY055069; A/Paris/2629/2009 (H1N1): GQ249337/GQ254714; A/Kobe/1/2009 (H1N1): GQ219577/GQ220733; A/Netherlands/602/2009 (H1N1): CY039527/CY039528; A/Beijing/01/2009 (H1N1): GQ183617/GQ183619; A/MexicoCity/MCIG01/2009 (H1N1): CY054296/CY054297; A/swine/Indiana/1726/1998 (H1N1): CY039925/CY039927; A/swine/Memphis/1/1990 (H1N1): CY035070/CY035072; A/swine/Iowa/24297/1991 (H1N1): CY027155/CY027157; A/swine/Shanghai/2/2005 (H1N1): EU502885/FJ789827. A/swine/Korea/SCJ01/2009(H1N1): HM189542/HM189430; A/swine/Iowa/930/01 (H1N2): AF455679/AF455695; A/swine/Minnesota/55551/00 (H1N2): AF 455678/AF455694. *Strain: Accession No. for HA/NA.

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