Maxillofac Plast Reconstr Surg.  2016 Aug;38(8):29. 10.1186/s40902-016-0076-y.

The effect of 4-hexylresorcinol on xenograft degradation in a rat calvarial defect model

• Kang YJ
• Noh JE
• Lee MJ
• Chae WS
• Lee SY
• Kim SG

• KMID: 2351663
• DOI: http://doi.org/10.1186/s40902-016-0076-y

Abstract

BACKGROUND
The objective of this study was to evaluate xenograft degradation velocity when treated with 4-hexylresorcinol (4HR).
METHODS
The scapula of a cow was purchased from a local grocery, and discs (diameter 8 mm, thickness 1 mm) were prepared by trephine bur. Discs treated with 4HR were used as the experimental group. Untreated discs were used as the control. X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), antibacterial test, endotoxin test, and scanning electron microscopy (SEM) were performed on the discs. In vivo degradation was evaluated by the rat calvarial defect model.
RESULTS
The XRD and FT-IR results demonstrated successful incorporation of 4HR into the bovine bone. The experimental disc showed antibacterial properties. The endotoxin test yielded results below the level of endotoxin contamination. In the SEM exam, the surface of the experimental group showed needle-shaped crystal and spreading of RAW264.7 cells. In the animal experiments, the amount of residual graft was significantly smaller in the experimental group compared to the control group (P = 0.003).
CONCLUSIONS
In this study, 4HR was successfully incorporated into bovine bone, and 4HR-incorporated bovine bone had antibacterial properties. In vivo experiments demonstrated that 4HR-incorporated bovine bone showed more rapid degradation than untreated bovine bone.

Keyword

4-Hexylresorcinol; Bovine; Bone graft; Degradation

MeSH Terms

Animal Experimentation
Animals
Heterografts*
Hexylresorcinol*
Microscopy, Electron, Scanning
Rats*
Scapula
Spectroscopy, Fourier Transform Infrared
Transplants
X-Ray Diffraction
Hexylresorcinol