J Korean Ophthalmol Soc.  1998 Sep;39(9):1996-2002.

Effects of Topically Applied Autologous Serum on Aqueous Biochemistry and Endothelial Healing Following Experimental Corneal Alkali Wounds

• Yoo JW
• Chung JH

Abstract

The effect of topically instilled autolohous serum on the healing of endothelium and change of aqueous chemical properties following alkali wounds were evaluated. New Zealand white rabbits, weighing 2.5KG, total 64(64 eyes), were used in this experiment. Standardized corneal alkali wounds were produced by applying a round filter paper of 5.5mm if diameter, soaked in 1N NaOH, to the central cornea for 60 seconds. Autologous serum in the treated group and balanced salt solution (BSS) in the control group were given 4 times per dat for 3 weeks. Endothelial defect areas and the concentrations of ascorbic acid, glucose and minerals in the aqueous humor were measured. Endothelial defect area was not significantly different between autologous serum-treated and control groups. The concentrations of ascorbic acid in the aqueous humor were aignificantly increased in the autologous serum-treated groups compared to the control groups. However, the concentrations of glucose, Na+, K+, Ca++ and Mg++ in the aqueous humor were not statistically different between two groups.

MeSH Terms

Alkalies*
Aqueous Humor
Ascorbic Acid
Biochemistry*
Cornea
Endothelium
Glucose
Minerals
Rabbits
Wounds and Injuries*
Alkalies
Ascorbic Acid
Glucose
Minerals