J Korean Soc Emerg Med.  2013 Aug;24(4):453-458.

Effectiveness of Polydeoxyribonucleotide (PDRN) Material on Murine Subcutaneous Laceration Wounds

• Kim JY
• Kim HJ
• Lee JB
• Seong SD
• Cho YS

Abstract

PURPOSE
To evaluate the effectiveness of polydeoxyribonucleotide (PDRN) material on murine subcutaneous laceration wounds.
METHODS
Subcutaneous laceration wounds were made on the back of mice. The mice were divided into two groups according to method of PDRN applied: Group I (control, general dressing, and management) and Group II (PDRN injection). We evaluated gross findings and histological findings for the groups.
RESULTS
A total of 18 mice (9 in the Suture group and 9 with Suture+PDRN) were enrolled. In the mean results of gross finding (5-point Likert scale), the mean gross findings for wounds in the PDRN group were significantly higher than the suture groups on post-operative day 4 and 7 (for day 4, Suture: 2, Suture+PDRN: 3.2; for day 7, Suture: 2.7, Suture+PDRN: 4.2; p<0.05). In the histological analysis of wounds in the Suture+PDRN group after 10 days, re-epithelization and granulation tissue formation were better than the Suture group. In terms of wound-healing grade, re-epithelization and inflammation were not different; however, in the Suture+PDRN group, more granulation tissue formation was noted compared to the Suture group (p<0.05). In addition, the expression of VEGF in the Suture+PDRN group significantly increased compared to the Suture group (Suture: 11170+/-2475, Suture + PDRN: 27243+/-6621, p<0.05).
CONCLUSION
The Suture+PDRN group can be used for the early treatment and histological healing of subcutaneous laceration wounds.

Keyword

PDRN; Polydeoxyribonucleotide; Subcutaneous; Laceration; Wound

MeSH Terms

Animals
Bandages
Granulation Tissue
Inflammation
Lacerations
Mice
Sutures
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factor A