J Korean Burn Soc.  2011 Dec;14(2):85-92.

Experimental Hind Limb & Inguinal-Femur Osteocutaneous Flap Model in Rats for Composite Tissue Transplantation

• Jeon YR
• Hong JW
• Kim YS
• Roh TS
• Lew DH
• Rah DK

Abstract

PURPOSE
Composite tissue allotransplantation (CTA) is a newly raised field as a treatment of severe body disfigurements. But in the point that it is transplant of functional organ not vital organ, it has limitation of using immunosuppressant for lifetime. Therefore, recent studies on CTA are focused on the reduction of risk of immunosuppression by inducing immunotolerance, developing new regimen and so on. So, appropriate experimental models to solve the problems are needed. We have performed CTA experiment using hind limb of rats which is frequently used CTA animal model. There were many trials and errors when actually conducting experiment. Hence the authors are to state the experiences in our own experiments.
METHODS
Total 13 Sprague Dawley rats (SD rats) were used. In 10 rats, hind limb allotransplantation were performed and in 3 rats, inguinal-femur osteocutanoeus flap operation were conducted.
RESULTS
Out of total 13 rats, on the day of operation 4 rats died, and 1dayafter operation 2 rats died. The remainder 7 rats were euthanized on the 3rd day after operation. Autophagy occurred in 3 rats. The dead experimental models were experienced in the early stage of study.
CONCLUSION
We could increase survival rate through appropriate anesthesia, maintaining body temperature, supplement of water and pain control.

Keyword

Composite tissue allotransplantation; Hind limb model; Inguinal-femur osteocutanoeus flap

MeSH Terms

Anesthesia
Animals
Autophagy
Body Temperature
Extremities
Immunosuppression
Models, Animal
Models, Theoretical
Rats
Rats, Sprague-Dawley
Survival Rate
Tissue Transplantation
Transplants