J Korean Assoc Maxillofac Plast Reconstr Surg.
2012 Nov;34(6):384-390.
In vivo Osteogenesis of Cultured Human Periosteal-derived Cells and Polydioxanone/Pluronic F127 Scaffold
- Affiliations
-
- 1Department of Oral and Maxillofacial Surgery, Gyeongsang National University School of Medicine, Institute of Health Sciences, Gyeongsang National University, Biomedical Center (BK21), Korea. surbyun@gsnu.ac.kr
- 2Department of Advanced Materials, College of Life Science and Nano Technology, Hannam University, Korea.
- 3Clinical Research Institute, Gyeongsang National University Hospital, Korea.
- 4Department of Obstetrics/Theriogenology and Biotechnology, College of Veterinary Medicine, Gyeongsang National University, Korea.
- 5Maxillofacial Center, On Hospital, Korea.
Abstract
- PURPOSE
The purpose of this study is to examine in vivo osteogenesis of cultured human periosteal-derived cells and polydioxanone/pluronic F127 scaffold.
METHODS
Two one-year-old miniature pigs were used in this study. 2x106 periosteal-derived cells in 1 mL medium were seeded by dropping the cell suspension into the polydioxanone/pluronic F127 scaffold. These cell-scaffold constructs were cultured in osteogenic Dulbecco's modified Eagle's medium for 7 days. Under general anesthesia with azaperone and tiletamine-zolazepam, the mandibular body and ramus of the pigs were exposed. Three bony defects were created. Polydioxanone/pluronic F127 scaffold with periosteal-derived cells and the scaffold only were implanted into each defect. Another defect was left empty. Twelve weeks after implantation, the animals were sacrificed.
RESULTS
New bone formation was clearly observed in the polydioxanone/pluronic F127 scaffold with periosteal-derived cells. Newly generated bone was also observed in the scaffold without periosteal-derived osteoblasts and empty defect, but was mostly limited to the periphery.
CONCLUSION
These results suggest that cultured human periosteal-derived cells have good osteogenic capacity in a polydioxanone/pluronic F127 scaffold, which provides a proper environment for the osteoblastic differentiation of these cells.
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