Restor Dent Endod.  2014 Nov;39(4):258-264. 10.5395/rde.2014.39.4.258.

Microorganism penetration in dentinal tubules of instrumented and retreated root canal walls. In vitro SEM study

• Al-Nazhan
• Al-Sulaiman A
• Al-Rasheed F
• Alnajjar F
• Al-Abdulwahab B
• Al-Badah A

• KMID: 2316905
• DOI: http://doi.org/10.5395/rde.2014.39.4.258

Abstract

OBJECTIVES
This in vitro study aimed to investigate the ability of Candida albicans (C. albicans) and Enterococcus faecalis (E. faecalis) to penetrate dentinal tubules of instrumented and retreated root canal surface of split human teeth.
MATERIALS AND METHODS
Sixty intact extracted human single-rooted teeth were divided into 4 groups, negative control, positive control without canal instrumentation, instrumented, and retreated. Root canals in the instrumented group were enlarged with endodontic instruments, while root canals in the retreated group were enlarged, filled, and then removed the canal filling materials. The teeth were split longitudinally after canal preparation in 3 groups except the negative control group. The teeth were inoculated with both microorganisms separately and in combination. Teeth specimens were examined by scanning electron microscopy (SEM), and the depth of penetration into the dentinal tubules was assessed using the SMILE view software (JEOL Ltd).
RESULTS
Penetration of C. albicans and E. faecalis into the dentinal tubules was observed in all 3 groups, although penetration was partially restricted by dentin debris of tubules in the instrumented group and remnants of canal filling materials in the retreated group. In all 3 groups, E. faecalis penetrated deeper into the dentinal tubules by way of cell division than C. albicans which built colonies and penetrated by means of hyphae.
CONCLUSIONS
Microorganisms can easily penetrate dentinal tubules of root canals with different appearance based on the microorganism size and status of dentinal tubules.

Keyword

Candida albicans; Dentinal tubules; Enterococcus faecalis; Root canal infection; Scanning electron microscopy

MeSH Terms

Candida albicans
Cell Division
Dental Pulp Cavity*
Dentin*
Enterococcus faecalis
Humans
Hyphae
Microscopy, Electron, Scanning
Tooth

Figure

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  Figure 1 The tool used to split the root into two halves.

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  Figure 2 Scanning electron microscope images of the negative control. (a) The dentin surface and the dentinal tubules were covered by pulp tissue; (b) Collagen fibers were seen inside the dentinal tubules.

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  Figure 3 Scanning electron microscope images of the positive control. (a) Deep penetration of E. faecalis into dentinal tubules. Note the size of the E. faecalis and the C. albicans; (b) The C. albicans blocked the opening of the dentinal tubules (arrow) mixed microorganisms.

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  Figure 4 (a) Scanning electron microscope images of the instrumented group showing E. faecalis inside the dentinal tubules; (b) It can be traced up to 244 µm - low magnification; (c) C. albicans inside the dentinal tubules; (d) Penetrated up to 184 µm.

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  Figure 5 Scanning electron microscope images of the re-treated group. (a) Note the depth of penetration of the dentinal tubules by C. albicans; (b) It can be traced up to 74 µm; (c) E. faecalis in dentinal tubules; (d) Penetrated up to 123 µm.

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