Obstet Gynecol Sci.  2014 Mar;57(2):144-150.

Comparison of estrogen receptor-alpha, progesterone receptor and calponin expression in gonadotrophin-releasing hormone agonist-sensitive and -resistant uterine fibroids

• Kim EH
• Kim JY
• Lee YH
• Chong GO
• Park JY
• Hong DG

Abstract

OBJECTIVE
This study was aimed to compare immunohistochemical expression of estrogen receptor (ER)-alpha, progesterone receptor (PR), and calponin in gonadotrophin-releasing hormone agonist (GnRH-a)-sensitive and -resistant uterine fibroids.
METHODS
We collected data retrospectively. The sensitive group consisted of women who had reduction in uterine volume greater than 40% following GnRH-a treatment. Uterine volume was either reduced by less than 10%, or was increased in the resistant group. A tissue microarray was constructed using formalin-fixed, paraffin-embedded tissues, 31 and 26 patients for the sensitive and resistant groups, respectively. Tissue sections were immunostained with antibodies against ER-alpha, PR, and calponin. The intensity and area of the immunohistochemical reactions were evaluated using a semi-quantitative scoring system. The Mann-Whitney U-test, Fisher's exact test, and Spearman's rank correlation test were used for analysis of data.
RESULTS
PR (P = 0.04) and calponin (P = 0.03) showed a significantly higher staining intensity in the resistant group than in the sensitive group. Both groups showed comparable expression of ER-alpha (P = 0.23). In correlation analysis between changes in uterine volume after GnRH-a therapy and clinicopathological factors, the immunohistochemical intensity of PR (P = 0.04) and calponin (P = 0.03) was significantly correlated with changes in uterine volume.
CONCLUSION
This study shows that GnRH-a resistance of uterine fibroids is not related to ER-alpha content, but the expression of PR and calponin is related with GnRH-a resistance.

Keyword

Calponin; Estrogen receptor; Gonadotropin-releasing hormone agonist; Leiomyoma; Progesterone receptor

MeSH Terms

Antibodies
Estrogens*
Female
Humans
Leiomyoma*
Progesterone*
Receptors, Progesterone*
Retrospective Studies
Antibodies
Estrogens
Progesterone
Receptors, Progesterone