Mycobiology.  2011 Jun;39(2):92-95.

Optimal Culture Conditions for Mycelial Growth of Lignosus rhinocerus

• Lai WH
• Siti Murni MJ
• Fauzi D
• Abas Mazni O
• Saleh NM

Abstract

Lignosus rhinocerus is a macrofungus that belongs to Polyporaceae and is native to tropical regions. This highly priced mushroom has been used as folk medicine to treat diseases by indigenous people. As a preliminary study to develop a culture method for edible mushrooms, the cultural characteristics of L. rhinocerus were investigated in a range of culture media under different environmental conditions. Mycelial growth of this mushroom was compared on culture media composed of various carbon and nitrogen sources in addition to C/N ratios. The optimal conditions for mycelial growth were 30degrees C at pH 6 and 7. Rapid mycelial growth of L. rhinocerus was observed on glucose-peptone and yeast extract peptone dextrose media. Carbon and nitrogen sources promoting mycelial growth of L. rhinocerus were glucose and potassium nitrate, respectively. The optimum C/N ratio was approximately 10 : 1 using 2% glucose supplemented as a carbon source in the basal media.

Keyword

Cultural conditions; Edible mushroom; Lignosus rhinocerus; Medicinal mushroom; Mycelial growth

MeSH Terms

Agaricales
Carbon
Cultural Characteristics
Culture Media
Glucose
Hydrogen-Ion Concentration
Medicine, Traditional
Nitrates
Nitrogen
Peptones
Polyporaceae
Potassium
Potassium Compounds
Yeasts
Carbon
Culture Media
Glucose
Nitrates
Nitrogen
Peptones
Potassium
Potassium Compounds