Mycobiology.  2010 Dec;38(4):310-315.

Isolation and Identification of Mushroom Pathogens from Agrocybe aegerita

• Choi IY
• Choi JN
• Sharma PK
• Lee WH

Abstract

Agrocybe aegerita is an important mushroom cultivated in Korea, with good feel and a peculiar fragrance. A. aegerita can be cultivated throughout the year using culture bottles but is more susceptible to contamination than other mushrooms. Twenty-two pathogens were isolated from the fruiting bodies and compost of A. aegerita, and seven isolates were isolated from Pleurotus ostreatus to compare with the A. aegerita isolates, collected from Gimje, Iksan, Gunsan of Chonbuk, and Chilgok of Gyeongbuk Province in 2009. These isolates were identified based on morphological and molecular characteristics. Of the 29 isolates, 26 were identified as Trichoderma spp. and the remaining three were Aspergillus spp., Mucor spp., and Penicillium spp. A phylogenetic analysis revealed that the 26 isolates of Trichoderma were divided into four taxa, namely T. harzianum, T. pleuroticola, T. longibrachiatum, and T. atroviride. Among the Trichoderma spp., 16 isolates (55.2%) were identified as T. harzianum, six as T. pleuroticola (20.7%), two as T. longibrachiatum, and the remaining two were T. atroviride.

Keyword

Agrocybe aegerita; Phylogenetic analysis; rDNA internal transcribed spacer sequence; Trichoderma spp.

MeSH Terms

Agaricales
Agrocybe
Aspergillus
Fruit
Korea
Mucor
Penicillium
Pleurotus
Soil
Trichoderma
Soil