Lab Anim Res.  2010 Jun;26(2):219-221. 10.5625/lar.2010.26.2.219.

Genetic Polymorphism of Jeju Horses by Microsatellite DNA Markers in Korea

• Lee JE
• Shin JH
• Yun YM
• Lee KK
• Lee H
• Kweon OK
• Yun YS
• Suh JG
• Shin NS
• Seong JK

• KMID: 2312069
• DOI: http://doi.org/10.5625/lar.2010.26.2.219

Abstract

We determined the genetic diversity and distance between Jeju and Thoroughbred horses by genotyping for 20 microsatellite loci consisting of (TG)n repetitive sequence. The expected heterozygosity ranged from 0.1 to 0.789 in the Jeju horses and from 0.505 to 0.824 in the Thoroughbred horses. Polymorphic information content (PIC) values ranged from 0.09 to 0.709 in the Jeju horses and 0.365 to 0.730 in Thoroughbred horses. There were no significant differences in heterozygosity and PIC values between Jeju and Thoroughbred horsesHowever, LEX035 was estimated relatively high heterozygosity (0.789) and PIC value 0.709) in Jeju horses and LEX050 was respectively 0.824, and 0.730 in the Thoroughbred horses. We may conclude that the genetic differentiation was low between Jeju and Thoroughbred horses. LEX 050, LEX055, LEX059 and LEX 063 could be used as geneticmarkers for differentiating Jeju from Thoroughbred horses.

Keyword

Jeju horses; genetic polymorphism; microsatellite DNA

MeSH Terms

DNA
Genetic Markers
Genetic Variation
Horses
Korea
Microsatellite Repeats
Polymorphism, Genetic
Repetitive Sequences, Nucleic Acid
DNA
Genetic Markers

Reference

Alford R. L.., Hammond H. A.., Coto I.., Caskey C. T.1994. Rapid and efficient resolution of parentage by amplification of short tandem repeats. Am. J. Hum. Genet. 55(1):190–195.

Bailey E.., Reid R.C.., Skow L.C.., Mathiason K.., Lear T.L.., McGuire T. C.1997. Linkage of the gene for equine combined immunodeficiency disease to microsatellite markers HTG8 and HTG4; synteny and FISH mapping to ECA9. Anim. Genet. 28(4):268–273.

Bowling A. T.., Eggleston-Stott M.L.., Byrns G.., Clark R.S.., Dileanis S.., Wictum E.1997. Validation of microsatellite markers for routine horse parentage testing. Anim. Genet. 28(4):247–252.

Buchanan F.C.., Adams L.J.., Littlejohn R.P.., Maddox J.F.., Crawford A. M.1994. Determination of evolutionary relationships among sheep breeds using microsatellites. Genomics. 22(2):397–403.

Coogle L.., Bailey E.1997. Equine dinucleotide repeat loci LEX049-LEX063. Anim. Genet. 28(5):378.

Coogle L.., Reid R.., Bailey E.1997. Equine dinucleotide repeat loci LEX034-LEX048. Anim. Genet. 28(4):309.

Dietrich W.., Katz H.., Lincoln S. E.., Shin H. S.., Friedman J.., Dracopoli N. C.., Lander E. S.1992. A genetic map of the mouse suitable for typing intraspecific crosses. Genetics. 131(2):423–447.

FitzSimmons N. N.., Moritz C.., Moore S. S.1995. Conservation and dynamics of microsatellite loci over 300 million years of marine turtle evolution. Mol. Biol. Evol. 12(3):432–440.

Forbes S. H.., Hogg J. T.., Buchanan F. C.., Crawford A. M.., Allendorf F. W.1995. Microsatellite evolution in congeneric mammals: domestic and bighorn sheep. Mol. Biol. Evol. 12(6):1106–1113.

Glowatzki-Mullis M. L.., Gaillard C.., Wigger G.., Fries R.1995. Microsatellite-based parentage control in cattle. Anim. Genet. 26(1):7–12.

Kim K. I.., Yang Y. H.., Lee S. S.., Park C.., Ma R.., Bouzat J. L.., Lewin H. A.1999. Phylogenetic relationships of Cheju horses to other horse breeds as determined by mtDNA D-loop sequence polymorphism. Anim. Genet. 30(2):102–108.

Pepin L.., Amigues Y.., Lepingle A.., Berthier J. L.., Bensaid A.., Vaiman D.1995. ).Sequence conservation of microsatellites between Bos taurus (cattle), Capra hircus (goat) and related species. Examples of use in parentage testing and phylogeny analysis. Heredity. 74(Pt1):53–61.

Shete S.., Tiwari H.., Elston R. C.2000. On Estimating the Heterozygosity and Polymorphism Information Content Value. Theor. Popul. Biol. 57(3):265–271.

Weissenbach J.., Gyapay G.., Dib C.., Vignal A.., Morissette J.., Millasseau P.., Vayssiex G.., Lathrop M.1992. A second-generation linkage map of the human genome. A second-generation linkage map of the human genome. Nature. 359(6398):794–801.