Korean J Med.
2001 Nov;61(5):479-488.
Hepatitis B virus (HBV) genotype in korean chronic HBV carriers: Whole HBV genome and it's nucleotide sequence by single polymerization chain reaction (PCR) Method
- Affiliations
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- 1Department of Internal Medicine, WonKwang University College of Medicine, Iksan, Korea.
- 2Department of Pathology, WonKwang University College of Medicine, Iksan, Korea.
- 3Department aof Clinical Pathology, WonKwang University College of Medicine, Iksan, Korea.
Abstract
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BACKGROUND: Hepatitis B virus (HBV) is major source of chronic liver disease in Korea. However this virus might have different nucleotide sequence according to races, different region, etc. Recently the novel method that allows sensitive amplification with dramatically decreased mis-incorporation has developed. We determined to get the major form of HBV nucleotide sequence from whole sequencing data of 26 Korean HBV carriers.
METHODS
HBV DNA were collected from 26 Korean chronic HBV carriers. We used the novel PCR with pfu for the amplification of HBV DNA, and specific primers were made with combination sequence bases of non-HBV part and HBV parts which were located head and tail in the virion. Then whole length of HBV were directly sequenced and analysed. RESULT: HBV DNA was consisted of 3215 bases in 20 cases of 26 Korean chronic HBV carriers. And the remainder had smaller or larger number due to deletion, insertion or both in pre-S2 and S gene. They were 99.03% homology of their nucleotide sequence and belong to genotype C. The variability of nucleotide sequence was significantly higher in the singly coding region (SCR) than doubly coding region (DCR), and also high in pre-S1 and pre-S2 gene among the DCR. Hot-spots were more frequently found in the SCR, pre-S1 and pre-S2 gene.
CONCLUSION
In Korean chronic HBV carriers, HBV is consisted of 3215 nucleotides, and belongs to genotype C. And it might exist one genotype with the variability in Korea.
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