Activities of alpha-D-Mannosidase and beta-D-Mannosidasein Patients with Liver Diseases

Park, Won Dae; Hwang, Jae Seok; Hur, Jung Wook; Ahn, Sung Hoon; Park, Soong Kook; Kwak, Chun Sik

Abstract

BACKGROUND/AIMS: We studied the activities of alpha- and beta-D-mannosidase to evaluate their diagnostic significance in liver diseases.
METHODS
One hundred fifty one patients with liver diseases were en rolled. The activities of the enzymes were measured by microquantitative spectrophotometry.
RESULTS
The activities of alpha-D-mannosidase isozymes (neutral and intermediate alpha-D-mannosidase) were signif icantly high in patients with icteric acute hepatitis (IAH)(48.4 +/-25.4, 17.6 +/-12.9, 12.2 +/-6.2), anicteric acute hepatitis (AAH)(29.9 +/-16.0, 11.9 +/-5.3, 10.2 +/-3.1), cholestatic hepatitis (ChH) (118.3 +/-20.6, 50.3 +/-12.2, 37.0 +/-5.5), icteric liver cirrhosis (ILC)(36.6 +/-13.5, 12.0 +/-7.8, 9.6 +/-4.3), ILC with hepa tocellular carcinoma (HCC)(39.0 +/-16.5, 15.1 +/-9.4, 11.1 +/-5.8). The activity of beta-D- mannosidase was also significantly high in patients with IAH (212 +/-36), icteric chronic hepatitis (ICH)(193 +/-25), ACH (196 +/-25), ChH (252 +/-38), ILC (188 +/-21), ILC with HCC (217 +/-55), and ALC with HCC (203 +/- 34).
CONCLUSIONS
The activities of alpha- and beta- D-mannosidase were significantly increased in liver diseases with jaundice, which was related to the increased permeability of the liver cell membrane and the leakage of the enzymes into the blood in large quantities. Serial measurement of these enzymes will be helpful to diagnose liver diseases and to understand the clinical conditions of the patients.