Korean J Androl.  2000 Dec;18(3):187-192.

Expression of Glucosamine-6-Phosphate Deaminase (GNPDA) in Mouse Epididymis

Affiliations
  • 1Department of Biology, Kyonggi University, Suwon, Korea.
  • 2Department of Urology, College of Medicine, Ajou University, Suwon, Korea.

Abstract

PURPOSE
To verify the expression of mouse homologue of glucosamine-6-phosphate deaminase (GNPDA) in mouse epididymis.
MATERIALS AND METHODS
GNPDA expression was examined in mouse epididymis using the polyclonal antibody raised against a synthetic oligopeptide of N-terminus of mouse homologue of GNPDA.
RESULTS
In Western blot, immunoreactive band of Mr. 33 kDa was detected in the cauda epididymal extracts. In immunostaining, positive signal was found in the cytoplasm and stereocillia of epithelial cells of cauda epididymal tubule, and interstitial cells of adult epididymis.
CONCLUSION
Expression of GNPDA in epididymis suggests that it possibly involved in absorption and intracellular digestion of luminal contents, and the remodeling of epithelia in mouse epididymis.

Keyword

GNPDA; Epididymis; Mouse

MeSH Terms

Absorption
Adult
Animals
Blotting, Western
Cytoplasm
Digestion
Epididymis*
Epithelial Cells
Humans
Male
Mice*
Phenobarbital
Phenobarbital
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