Korean J Thorac Cardiovasc Surg.  1997 Feb;30(2):119-124.

Effects of verapamil in cardioplegic perfusates on the ischemic myocardium in isolated rat heart

Affiliations
  • 1Department of Thoracic and Cardiovascular Surgery, College of Medicine, Kyung Hee University, Korea.

Abstract

Using isolated rat heart preparations, we observed the protective effects of verapamil cardioplegia on ischemic myocardial injury. Isolated rat hearts were subjected to global ischemia at 25degrees C. Twenty four isolated Sprague Dawley rat hearts underwent 30 minutes of the retrograde nonworking perfusion with Krebs-Henseleit buffer solution followed by 25degrees C cardioplegic solution(St. Thomas' Hospital Cardioplegic Solution) for 60 minutes. Before ischemic arrest, rat hearts were treated with cold cardioplegic solution in control group(n=12) and cold cardioplegic solution with verapamil(1mg/L) in experimental group(n=12). After 60 minutes of ischemia, hemodynamic and biochemical parameters such as heart rate, left ventricular pressure(LVP), +dp/dt max, coronary flow and creatine phosphokinase(CPK) were measured before giving cardioplegia and 30 minutes after reperfusion. Verapamil group exhibited greater recovery of heart rate, LVP, +dp/dt max, coronary flow and CPK than control group(p<0.05).

Keyword

Verapamil; Isolated rat heart

MeSH Terms

Animals
Cardioplegic Solutions
Creatine
Heart Arrest, Induced
Heart Rate
Heart*
Hemodynamics
Ischemia
Myocardium*
Perfusion
Rats*
Reperfusion
Verapamil*
Cardioplegic Solutions
Creatine
Verapamil
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