Korean J Otolaryngol-Head Neck Surg.  2000 Aug;43(8):836-843.

Detection of Human Papillomavirus and Epstein-Barr Virus in Sinonasal Inverted Papillomas and Its Correlation with p53 and Proliferating Cell Nuclear Antigen Expression

• Cho JH
• Suh BD
• Jun BC
• Chang HS
• Yang MJ
• Yoon HR
• Ro WY

Abstract

BACKGROUND AND OBJECTIVES: Sinonasal inverted papillomas are benign but topographically aggressive neoplasms that have a high recurrence rate and seem to be associated with malignancy. The etiology of inverted papilloma remains unknown, but some hypotheses suggest that nasal polyps proliferation and chronic inflammation are due to allergy or various infectious lesions. This study was to elucidate the biological characteristics and the role of human papillomavirus (HPV) and Ebstein -Barr virus (EBV) and the expression of p53 protein and proliferating cell nuclear antigen (PCNA) in sinonasal inverted papillomas.
MATERIALS AND METHODS
We examined 26 specimens from 26 individuals with normal nasal mucosae (n=10) and inverted papillomas (n=16) to determine the occurance of HPV and EBV infection and the expression of p53 protein and PCNA.
RESULTS
Of the 16 Inverted papillomas, HPV DNA was detected in eight cases, HPV 18 was detected in two cases (18%), HPV 16 and HPV 33 were both found in every case (6%), HPV 6 and HPV 16 were coinfected in one case (6%), and other types were found in 3 cases. HPV DNA was not detected in the normal nasal mucosae. EBV DNA was detected in 10 cases (62%) out of 16 inverted papillomas ancl in two cases (20%) of 10 normal nasal mocosae. The altered p53 protein expression was observed in four cases (25%), and positive PCNA staining was detected in four cases (25%) out of 16 inverted papillomas. One positive PCNA staining was detected among 10 normal mucosae. The mean PC10 index was 16.0% in the inverted papillomas group and 4.1% in normal nasal mucosae group.
CONCLUSION
An inverse correlation may exist between oncogenic HPV infection and p53 alteration in sinonasa1 inverted papillomas.

Keyword

Inverted papillomas; p53; Proliferating cell nuclear antigen (PCNA)

MeSH Terms

DNA
Epstein-Barr Virus Infections
Herpesvirus 4, Human*
Human papillomavirus 16
Human papillomavirus 18
Human papillomavirus 6
Humans*
Hypersensitivity
Inflammation
Mucous Membrane
Nasal Mucosa
Nasal Polyps
Papilloma, Inverted*
Population Characteristics
Proliferating Cell Nuclear Antigen*
Recurrence
DNA
Proliferating Cell Nuclear Antigen