Korean J Otolaryngol-Head Neck Surg.  1998 Sep;41(9):1118-1121.

Bone Resorption of Cultured Middle Ear Cholesteatomatous Epithelium-In Vitro Study

• Chang HJ
• Chung JW
• Lee SH
• Chang Y
• Kim HJ
• Yoon TH

Abstract

BACKGROUND AND OBJECTIVES: Bone resorption is an important cause of complications of chronic cholesteatomatous otitis media. Authors cultured the calvarial bone of fetal rat and measured the calcium release by keratinocytes and fibroblasts from cholesteatoma and postauricular skin (PAS).
MATERIALS AND METHODS
Keratinocytes and subepithelial fibroblasts were cultured from cholesteatoma and PAS. The pregnant rats were sacrificed 24 hours after subcutaneous injection of Ca45 and the fetal calvarial bones were cultured with or without the supernatants of keratinocytes and fibroblast culture. The amount of released radioactive calcium was analyzed using beta-ray counter.
RESULTS
The percentage (%) of calcium release was 28.95+/-4.0% in the control and 31.86+/-3.0% in the supernatant of PAS keratinocytes. In the cholesteatomatous keratinocytes, the released calcium was 34.99+/-6.1% and significantly greater than the values of control and PAS. Using the supernatants from the fibroblast culture, cholesteatoma and PAS showed higher calcium release than the control, but there was no significant difference between the two tissues.
CONCLUSIONS
Through this study, authors showed the in-vitro bone resorption by cholesteatoma and concluded that cholesteatomatous epithelial cells can be involved in the bone resorption.

Keyword

Bone resorption; Fetal rat calvarial culture; Cholesteatoma; Calcium release

MeSH Terms

Animals
Bone Resorption*
Calcium
Cholesteatoma
Ear, Middle*
Epithelial Cells
Fibroblasts
Injections, Subcutaneous
Keratinocytes
Otitis Media
Rats
Skin
Calcium