Korean J Otolaryngol-Head Neck Surg.  1998 Mar;41(3):385-391.

Immunohistochemical Classification and Detection of Epstein-Barr Virus of Cervical Nodal Malignant Lymphoma

• Suh JS
• Kim YD
• Lee JJ
• Choi CG
• Kim DS
• Lee TY

Abstract

BACKGROUND AND OBJECTIVES: Malignant lymphoma is the most common type of cancer in the immunologic system. There have been many reports about malignant lymphoma, but etiologies and prognosis of malignant lymphoma remain controversial. This study was designed to carry out an immunohistochemical classification of malignant lymphoma and to define the relationship between Epstein-Barr virus (EBV) and malignant lymphoma by using the polymerase chain reaction (PCR), which is known to be more sensitive than than any other methods for detection of EBV.
MATERIALS AND METHODS
Thirty six cases of cervical nodal non-Hodgkin's lymphoma tissue and twenty five cases of normal neck node tissue were used.
RESULTS
We observed 72.7% (26 cases/36 cases) B-cell lineage expression and 27.8% (10 cases/36 cases) T-cell lineage expression in the cervical nodal of non-Hodgkin's lymphoma. The detection rate of EBV in the malignant lymphoma tissue was 27.8% (10 cases/36 cases) and 7.1% (2 cases/36 cases) in the normal neck node tissue. Of the B-cell lineage expression, EBV was detected in 6 cases (23.1%) and 4 cases (40.0%) in T-cell league expression. There was no statistical significance between the malignant lymphoma group and the normal group in the detection rate of EBV. However, the detection rate of EBV of the T-cell malignant lymphoma group was significantly higher compared with the normal group (p<0.05).
CONCLUSION
These results suggest that the T-cell expression group of cervical nodal non-Hodgkin's lymphoma may be related to EBV.

Keyword

Malignant lymphoma; Immunohistochemical classification; Polymerase chain reaction(PCR); Epstein-Barr virus(EBV)

MeSH Terms

B-Lymphocytes
Classification*
Herpesvirus 4, Human*
Lymphoma*
Lymphoma, Non-Hodgkin
Neck
Polymerase Chain Reaction
Prognosis
T-Lymphocytes