Korean J Obstet Gynecol.  2009 Jul;52(7):724-733.

Ovarian cancer related gene targeting with large circular antisense library

• Doh KO
• Chun GS
• Sim JC
• Yang HS

Abstract

OBJECTIVE
The aim of this study is to find out the genes which are related to ovarian cancer cell growth using large circular antisense library.
METHODS
Clones for antisense library were uni-directionally sub-cloned into pBS SK (-) vector. LC-antisense molecules were then purified from the culture supernatants of the bacterial competent cells superinfected with M13K07 helper bacteriophages. The LC-antisense library to 240 unigene clone was constructed and utilized in the identification of genes functionally involved in the growth of ovarian cancer cells.
RESULTS
The 17 numbers out of the 240 numbers of the antisense library exerted a marked inhibitory effect on the growth of SK-OV 3.
CONCLUSION
The putative functional categorization of each gene was then conducted via public databases. These candidates may be used as target genes for drug development or adjuvant of conventional chemotherapeutic drugs.

Keyword

Ovarian neoplasms; Large circular antisense DNA; Gene therapy

MeSH Terms

Bacteriophages
Clone Cells
Estrone
Gene Targeting
Genetic Therapy
Ovarian Neoplasms
Estrone