Korean J Obstet Gynecol.  2005 Apr;48(4):857-866.

Factor V Leiden mutation in Korean women with pregnancy-induced hypertension

• Cho YK
• Ko JK
• Lee CM
• Choi H
• Kim BR
• Lee HK

Abstract

OBJECTIVE
The purposes of this study was to evaluate the frequency of Leiden mutation (missense mutation in the factor V gene at exon 10, 1691 CGA to CAA) in Korean women with well characterized pregnancy-induced hypertension (PIH) compared with normotensive gravid women.
METHODS
Genomic DNA from 121 PIH cases and 98 normotensive pregnant control cases were used for polymerase chain reaction (PCR). To genotype Leiden mutation (missense mutation in the factor V gene, exon 10 (1691 G to A)), primers (5'-TGC CCA GTG CTT AAC AAG ACC A-3', 5'-TGT TAT CAC ACT GGT GCT AA-3') were employed to make 267 base pair (bp) PCR product. There was an initial denaturation at 94 degrees C 5 min, followed by 30 cycles of one minute at 94 degrees C, one minute at 55 degrees C, and one minute at 72 degrees C. A 267 bp PCR product was further digested with Mnl I for 2 hour at 37 degrees C and analysed through 12% polyacrylamide gel electrophoresis to determine genotype. Allele 1691G yielded 37 bp, 67 bp, 163 bp fragment and allele 1691A yielded 67 bp, 200 bp fragment.
RESULTS
We examined the genotypes of factor V of 121 Korean women with pregnant induced hypertension and 98 normal pregnant women. None of the 219 Korean women carried the factor V Leiden mutation.
CONCLUSION
The factor V Leiden mutation is absent and not a common cause of PIH in Korean women.

Keyword

Pregnancy induced hypertension (PIH); Factor V (FV); Leiden mutation; Polymerase chain reaction (PCR); Restriction fragment length polymorphism (RFLP)

MeSH Terms

Alleles
Base Pairing
DNA
Electrophoresis, Polyacrylamide Gel
Exons
Factor V*
Female
Genotype
Humans
Hypertension
Hypertension, Pregnancy-Induced*
Polymerase Chain Reaction
Pregnancy
Pregnant Women
DNA
Factor V