Korean J Obstet Gynecol.  2003 May;46(5):938-945.

Mutational Analysis of Growth Differentiation Factor-9 Gene in Korean Women with Premature Ovarian Failure

• Gu HJ
• Kim YM
• Jang DW
• Lee JY
• Sohn IS
• Kim SN
• Park KH
• Zinn AR

Abstract

The clinical models for studying ovary-determining genes may be premature ovarian failure (POF). POF is a condition causing amenorrhea, hypoestrogenism, and elevated gonadotropins in women under 40 years old. FSH receptor, LH receptor, inhibin, GDF-9 (growth differentiation factor-9), BMP-15 (bone morphogenetic protein-15), DIAPH2 (diaphanous gene) and XPNPEP2 (X-prolyl aminopeptidase) genes were proposed as a possible candidate gene, but until recently, only mutations in FSH receptor, LH receptor and inhibin genes have been identified in POF patients. Therefore mutation screening of another POF gene necessary to reveal the principal causative genes of POF.
OBJECTIVE
The present study was performed to analyze the mutation of GDF-9 gene in Korean patient with POF and to investigate whether mutation of these gene is a likely main cause of POF.
METHODS
Eighty-six women with POF were studied and thirty-six normal women were enrolled as control. Mutation screening of these genes were performed by denaturing HPLC and were confirmed by automatic sequencing.
RESULTS
Three different mutations of GDF-9 gene were identified in Korean women with POF; Arg3Cys mutation in one patient, Leu40Val mutation in one patient, Asp57Tyr mutation in 10 patients and 5 normal controls. Arg3Cys mutation and Leu40Val mutation were likely cause of disease. Frequencies of Arg3Cys mutation and Leu40Val mutation were 1.2%, respectively. Asp57Tyr mutation was common polymorphism in Korean women. All mutations was a novel mutation found in the present study.
CONCLUSION
POF was resulted by mutations of GDF-9 gene, but mutations of GDF-9 gene are not likely main causes of POF because of low frequency of mutations.

Keyword

Premature ovarian failure; GDF-9 gene; dHPLC

MeSH Terms

Adult
Amenorrhea
Bone Morphogenetic Protein 15
Chromatography, High Pressure Liquid
Female
Gonadotropins
Growth Differentiation Factor 9
Humans
Inhibins
Mass Screening
Primary Ovarian Insufficiency*
Receptors, FSH
Receptors, LH
Bone Morphogenetic Protein 15
Gonadotropins
Growth Differentiation Factor 9
Inhibins
Receptors, FSH
Receptors, LH