Korean J Orthod.  1994 Mar;24(1):115-123.

Effects of carbonic anhydrase inhibitors on the lps-induced bone resorption in vitro

• Park YH
• Cha KS

Abstract

To study bone resorption mechanism, effect of LPS on the 45Ca release from fetal rat ulnae and radii, and effects of carbonic anhydrase inhibitors on the LPS-induced bone resorption in organ culture were studied. Ulnae and radii were removed from 19 day old fetal rats, prelabelled by subcutaneous injection of 200micronCi 45CaCl2 into their mother on the 17th day of gestation. Radioactivities of 45Ca released into media were determined after 24, 48 and 72 hours. Effects of LPS and carbonic anhydrase inhibitors were observed by the ratio of % release of 45Ca between paired control and experimental group. The observed results were as follows: 1. LPS(1microng/ml) supplemented in media for 72hours increased the 45Ca release significantly after 48 and 72 hours of culture and LPS(10microng/ml) increased the 45Ca release significantly after 72 hours of culture. 2. LPS-induced 45Ca release was not inhibited significantly by 1mM sulfanilamide but inhibited significantly by 10mM sulfanilamide after 48 and 72 hours of culture. 3. LPS-induced 45Ca release was not inhibited significantly by 0.1mM dichlorphenamide but inhibited significantly by 1mM dichlorphenamide after 48 and 72 hours of culture. 4. LPS-induced 45Ca release was not inhibited significantly by 1mM acetazolamide but inhibited significantly by 5mM acetazolamide after 72 hours of culture.

Keyword

Carbonic Arhydrase Inhibitor; Bone resorption

MeSH Terms

Acetazolamide
Animals
Bone Resorption*
Carbon*
Carbonic Anhydrase Inhibitors*
Carbonic Anhydrases*
Dichlorphenamide
Humans
Injections, Subcutaneous
Mothers
Organ Culture Techniques
Pregnancy
Radioactivity
Rats
Ulna
Acetazolamide
Carbon
Carbonic Anhydrase Inhibitors
Carbonic Anhydrases
Dichlorphenamide